Seroatlas · Human Serome Atlas

GTF2H4

General transcription factor IIH subunit 4

Also known as: P52, TF2H4_HUMAN, TFB2, TFIIH

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q92759
Gene
GTF2H4
Ensembl
ENSG00000213780
Chromosome
6
Canonical length
462 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nuclear speckles

OverviewNCBI Gene

Enables RNA polymerase II general transcription initiation factor activity. Involved in transcription by RNA polymerase II. Located in nuclear speck. Part of core TFIIH complex portion of holo TFIIH complex and transcription factor TFIID complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

462 residues, UniProt reviewed canonical sequence.

>Q92759|GTF2H4
     1  MESTPSRGLN RVHLQCRNLQ EFLGGLSPGV LDRLYGHPAT CLAVFRELPS LAKNWVMRML
    61  FLEQPLPQAA VALWVKKEFS KAQEESTGLL SGLRIWHTQL LPGGLQGLIL NPIFRQNLRI
   121  ALLGGGKAWS DDTSQLGPDK HARDVPSLDK YAEERWEVVL HFMVGSPSAA VSQDLAQLLS
   181  QAGLMKSTEP GEPPCITSAG FQFLLLDTPA QLWYFMLQYL QTAQSRGMDL VEILSFLFQL
   241  SFSTLGKDYS VEGMSDSLLN FLQHLREFGL VFQRKRKSRR YYPTRLAINL SSGVSGAGGT
   301  VHQPGFIVVE TNYRLYAYTE SELQIALIAL FSEMLYRFPN MVVAQVTRES VQQAIASGIT
   361  AQQIIHFLRT RAHPVMLKQT PVLPPTITDQ IRLWELERDR LRFTEGVLYN QFLSQVDFEL
   421  LLAHARELGV LVFENSAKRL MVVTPAGHSD VKRFWKRQKH SS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GTF2H4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.3
Highest tissue expression
21 nTPM

Expression across tissuesHPA

Tissue

  • liver: 21 nTPM
  • cerebellum: 20 nTPM
  • kidney: 14 nTPM
  • esophagus: 14 nTPM
  • pituitary gland: 13 nTPM
  • thyroid gland: 13 nTPM

Single-cell type

  • tuft cells: 12 nCPM
  • astrocytes: 9 nCPM
  • brain inhibitory neurons: 8.1 nCPM
  • brain excitatory neurons: 7.1 nCPM
  • oligodendrocyte progenitor cells: 7.1 nCPM
  • bergmann glia: 6.7 nCPM

Immune cell

  • myeloid DC: 2.5 nTPM
  • naive CD8 T-cell: 2.2 nTPM
  • T-reg: 2.1 nTPM
  • basophil: 1.9 nTPM
  • memory CD8 T-cell: 1.4 nTPM
  • memory B-cell: 1.3 nTPM

Brain region

  • white matter: 10 nTPM
  • cerebral cortex: 7.8 nTPM
  • basal ganglia: 6.9 nTPM
  • hypothalamus: 6.3 nTPM
  • spinal cord: 6.2 nTPM
  • cerebellum: 6 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.81
gnomAD pLI
0
gnomAD missense Z
1.1
DepMap mean gene effect
-0.71
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 9% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Transcription factor TFIIH subunit p52/Tfb2
  • Transcription factor Tfb2, C-terminal domain
  • Transcription factor Tfb2
  • Transcription factor Tfb2 (p52) C-terminal domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of GTF2H4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads GTF2H4 as an antibody target. Whether an autoantibody or antibody against GTF2H4 could matter depends on whether native GTF2H4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

GTF2H4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label GTF2H4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/GTF2H4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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