Seroatlas · Human Serome Atlas

GTF2H3

General transcription factor IIH subunit 3

Also known as: BTF2, P34, TF2H3_HUMAN, TFIIH

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q13889
Gene
GTF2H3
Ensembl
ENSG00000111358
Chromosome
12
Canonical length
308 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm

OverviewNCBI Gene

This gene encodes a member of the TFB4 family. The encoded protein is a subunit of the core-TFIIH basal transcription factor and localizes to the nucleus. The encoded protein is involved in RNA transcription by RNA polymerase II and nucleotide excision repair and associates with the Cdk-activating kinase complex. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 14. [provided by RefSeq, Dec 2012]

Canonical amino-acid sequenceUniProt

308 residues, UniProt reviewed canonical sequence.

>Q13889|GTF2H3
     1  MVSDEDELNL LVIVVDANPI WWGKQALKES QFTLSKCIDA VMVLGNSHLF MNRSNKLAVI
    61  ASHIQESRFL YPGKNGRLGD FFGDPGNPPE FNPSGSKDGK YELLTSANEV IVEEIKDLMT
   121  KSDIKGQHTE TLLAGSLAKA LCYIHRMNKE VKDNQEMKSR ILVIKAAEDS ALQYMNFMNV
   181  IFAAQKQNIL IDACVLDSDS GLLQQACDIT GGLYLKVPQM PSLLQYLLWV FLPDQDQRSQ
   241  LILPPPVHVD YRAACFCHRN LIEIGYVCSV CLSIFCNFSP ICTTCETAFK ISLPPVLKAK
   301  KKKLKVSA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GTF2H3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.33
Highest tissue expression
16 nTPM

Expression across tissuesHPA

Tissue

  • lymph node: 16 nTPM
  • rectum: 15 nTPM
  • tonsil: 15 nTPM
  • thymus: 15 nTPM
  • cerebral cortex: 15 nTPM
  • breast: 15 nTPM

Single-cell type

  • erythrocyte progenitors: 68 nCPM
  • early primary spermatocytes: 49 nCPM
  • cytotrophoblasts: 47 nCPM
  • differentiating spermatogonia: 42 nCPM
  • megakaryocyte-erythroid progenitors: 39 nCPM
  • megakaryocyte progenitors: 39 nCPM

Immune cell

  • basophil: 12 nTPM
  • NK-cell: 11 nTPM
  • naive CD4 T-cell: 8.7 nTPM
  • memory CD8 T-cell: 8.1 nTPM
  • naive CD8 T-cell: 8 nTPM
  • gdT-cell: 7.6 nTPM

Brain region

  • white matter: 18 nTPM
  • pons: 17 nTPM
  • medulla oblongata: 17 nTPM
  • thalamus: 17 nTPM
  • cerebral cortex: 16 nTPM
  • basal ganglia: 16 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.04
gnomAD pLI
0
gnomAD missense Z
0.65
DepMap mean gene effect
-0.89
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of GTF2H3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads GTF2H3 as an antibody target. Whether an autoantibody or antibody against GTF2H3 could matter depends on whether native GTF2H3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

GTF2H3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label GTF2H3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/GTF2H3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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