Seroatlas · Human Serome Atlas

MNAT1

CDK-activating kinase assembly factor MAT1

Also known as: MAT1, MAT1_HUMAN, RNF66

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P51948
Gene
MNAT1
Ensembl
ENSG00000020426
Chromosome
14
Canonical length
309 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm

OverviewNCBI Gene

The protein encoded by this gene, along with cyclin H and CDK7, forms the CDK-activating kinase (CAK) enzymatic complex. This complex activates several cyclin-associated kinases and can also associate with TFIIH to activate transcription by RNA polymerase II. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Canonical amino-acid sequenceUniProt

309 residues, UniProt reviewed canonical sequence.

>P51948|MNAT1
     1  MDDQGCPRCK TTKYRNPSLK LMVNVCGHTL CESCVDLLFV RGAGNCPECG TPLRKSNFRV
    61  QLFEDPTVDK EVEIRKKVLK IYNKREEDFP SLREYNDFLE EVEEIVFNLT NNVDLDNTKK
   121  KMEIYQKENK DVIQKNKLKL TREQEELEEA LEVERQENEQ RRLFIQKEEQ LQQILKRKNK
   181  QAFLDELESS DLPVALLLAQ HKDRSTQLEM QLEKPKPVKP VTFSTGIKMG QHISLAPIHK
   241  LEEALYEYQP LQIETYGPHV PELEMLGRLG YLNHVRAASP QDLAGGYTSS LACHRALQDA
   301  FSGLFWQPS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MNAT1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.43
Highest tissue expression
76 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 76 nTPM
  • tongue: 61 nTPM
  • parathyroid gland: 52 nTPM
  • blood vessel: 31 nTPM
  • retina: 27 nTPM
  • kidney: 25 nTPM

Single-cell type

  • myonuclei: 593 nCPM
  • distal convoluted tubule cells: 284 nCPM
  • renal connecting tubule cells: 248 nCPM
  • podocytes: 241 nCPM
  • renal collecting duct intercalated cells: 235 nCPM
  • gonadotrophs: 221 nCPM

Immune cell

  • plasmacytoid DC: 47 nTPM
  • NK-cell: 35 nTPM
  • memory B-cell: 27 nTPM
  • eosinophil: 22 nTPM
  • naive B-cell: 20 nTPM
  • naive CD8 T-cell: 18 nTPM

Brain region

  • cerebral cortex: 46 nTPM
  • white matter: 45 nTPM
  • thalamus: 44 nTPM
  • cerebellum: 44 nTPM
  • basal ganglia: 42 nTPM
  • hypothalamus: 40 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.52
gnomAD pLI
0
gnomAD missense Z
0.72
DepMap mean gene effect
-0.55
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MNAT1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MNAT1 as an antibody target. Whether an autoantibody or antibody against MNAT1 could matter depends on whether native MNAT1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MNAT1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MNAT1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MNAT1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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