E2F3

Protein identityUniProt · HPA

UniProt accession

O00716

Gene

E2F3

Ensembl

ENSG00000112242

Chromosome

6

Canonical length

465 aa

Protein class

Cancer-related genes, Predicted intracellular proteins, Transcription factors

Subcellular location

Nucleoplasm

OverviewNCBI Gene

This gene encodes a member of a small family of transcription factors that function through binding of DP interaction partner proteins. The encoded protein recognizes a specific sequence motif in DNA and interacts directly with the retinoblastoma protein (pRB) to regulate the expression of genes involved in the cell cycle. Altered copy number and activity of this gene have been observed in a number of human cancers. There are pseudogenes for this gene on chromosomes 2 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

Canonical amino-acid sequenceUniProt

465 residues, UniProt reviewed canonical sequence.

>O00716|E2F3
     1  MRKGIQPALE QYLVTAGGGE GAAVVAAAAA ASMDKRALLA SPGFAAAAAA AAAPGAYIQI
    61  LTTNTSTTSC SSSLQSGAVA AGPLLPSAPG AEQTAGSLLY TTPHGPSSRA GLLQQPPALG
   121  RGGSGGGGGP PAKRRLELGE SGHQYLSDGL KTPKGKGRAA LRSPDSPKTP KSPSEKTRYD
   181  TSLGLLTKKF IQLLSQSPDG VLDLNKAAEV LKVQKRRIYD ITNVLEGIHL IKKKSKNNVQ
   241  WMGCSLSEDG GMLAQCQGLS KEVTELSQEE KKLDELIQSC TLDLKLLTED SENQRLAYVT
   301  YQDIRKISGL KDQTVIVVKA PPETRLEVPD SIESLQIHLA STQGPIEVYL CPEETETHSP
   361  MKTNNQDHNG NIPKPASKDL ASTNSGHSDC SVSMGNLSPL ASPANLLQQT EDQIPSNLEG
   421  PFVNLLPPLL QEDYLLSLGE EEGISDLFDA YDLEKLPLVE DFMCS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against E2F3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.57

Highest tissue expression

19 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.35

gnomAD pLI

0.95

gnomAD missense Z

1.59

DepMap mean gene effect

-0.22

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 9% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• G1/S transition of mitotic cell cycle
• negative regulation of fat cell proliferation
• positive regulation of cell population proliferation
• positive regulation of transcription by RNA polymerase II
• positive regulation of vascular associated smooth muscle cell apoptotic process
• protein import into nucleus
• regulation of transcription by RNA polymerase II
• transcription initiation at RNA polymerase II promoter

Molecular functions

• cis-regulatory region sequence-specific DNA binding
• DNA-binding transcription activator activity
• DNA-binding transcription activator activity, RNA polymerase II-specific
• DNA-binding transcription factor activity
• DNA-binding transcription factor activity, RNA polymerase II-specific
• protein dimerization activity
• RNA polymerase II cis-regulatory region sequence-specific DNA binding
• sequence-specific DNA binding
• sequence-specific double-stranded DNA binding

Cellular components

• chromatin
• cytosol
• nucleoplasm
• nucleus
• RNA polymerase II transcription regulator complex

Protein domainsUniProt · Pfam · InterPro

• E2F/DP family, winged-helix DNA-binding domain
• E2F Family
• E2F transcription factor, CC-MB domain
• Winged helix-like DNA-binding domain superfamily
• Winged helix DNA-binding domain superfamily
• E2F-DP heterodimerization region
• E2F/DP family winged-helix DNA-binding domain
• E2F transcription factor CC-MB domain

KeywordsUniProt

• Activator
• Cell cycle
• DNA-binding
• Nucleus
• Transcription
• Transcription regulation

InteractionsUniProt · HPA

Protein binding partners of E2F3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• RB1
• EAPP
• HELLS