Seroatlas · Human Serome Atlas

DNAJC21

DnaJ homolog subfamily C member 21

Also known as: DJC21_HUMAN, DNAJA5, GS3, JJJ1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q5F1R6
Gene
DNAJC21
Ensembl
ENSG00000168724
Chromosome
5
Canonical length
531 aa
Protein class
Disease related genes, Human disease related genes, Predicted intracellular proteins
Subcellular location
Nucleoli,Cytosol

OverviewNCBI Gene

This gene encodes a member of the DNAJ heat shock protein 40 family of proteins that is characterized by two N-terminal tetratricopeptide repeat domains and a C-terminal DNAJ domain. This protein binds the precursor 45S ribosomal RNA and may be involved in early nuclear ribosomal RNA biogenesis and maturation of the 60S ribosomal subunit. Mutations in this gene result in Bone marrow failure syndrome 3. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Feb 2017]

Canonical amino-acid sequenceUniProt

531 residues, UniProt reviewed canonical sequence.

>Q5F1R6|DNAJC21
     1  MKCHYEALGV RRDASEEELK KAYRKLALKW HPDKNLDNAA EAAEQFKLIQ AAYDVLSDPQ
    61  ERAWYDNHRE ALLKGGFDGE YQDDSLDLLR YFTVTCYSGY GDDEKGFYTV YRNVFEMIAK
   121  EELESVLEEE VDDFPTFGDS QSDYDTVVHP FYAYWQSFCT QKNFAWKEEY DTRQASNRWE
   181  KRAMEKENKK IRDKARKEKN ELVRQLVAFI RKRDKRVQAH RKLVEEQNAE KARKAEEMRR
   241  QQKLKQAKLV EQYREQSWMT MANLEKELQE MEARYEKEFG DGSDENEMEE HELKDEEDGK
   301  DSDEAEDAEL YDDLYCPACD KSFKTEKAMK NHEKSKKHRE MVALLKQQLE EEEENFSRPQ
   361  IDENPLDDNS EEEMEDAPKQ KLSKKQKKKK QKPAQNYDDN FNVNGPGEGV KVDPEDTNLN
   421  QDSAKELEDS PQENVSVTEI IKPCDDPKSE AKSVPKPKGK KTKDMKKPVR VPAEPQTMSV
   481  LISCTTCHSE FPSRNKLFDH LKATGHARAP SSSSLNSATS SQSKKEKRKN R

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DNAJC21 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.49
Highest tissue expression
83 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 83 nTPM
  • testis: 67 nTPM
  • tongue: 48 nTPM
  • heart muscle: 36 nTPM
  • placenta: 25 nTPM
  • liver: 25 nTPM

Single-cell type

  • late primary spermatocytes: 188 nCPM
  • alveolar cells type 2: 147 nCPM
  • urothelial cells: 109 nCPM
  • breast lactating cells: 101 nCPM
  • suprabasal keratinocytes: 98 nCPM
  • transitional alveolar cells: 94 nCPM

Immune cell

  • NK-cell: 6.2 nTPM
  • eosinophil: 4.4 nTPM
  • T-reg: 4.1 nTPM
  • memory B-cell: 3.3 nTPM
  • memory CD4 T-cell: 2.9 nTPM
  • plasmacytoid DC: 2.9 nTPM

Brain region

  • cerebral cortex: 16 nTPM
  • choroid plexus: 16 nTPM
  • hypothalamus: 15 nTPM
  • medulla oblongata: 15 nTPM
  • spinal cord: 15 nTPM
  • white matter: 15 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about DNAJC21.

Disease | AllUniProt

Conditions DNAJC21 is implicated in, by any mechanism.

Disease | GeneticClinVar

49 pathogenic / likely-pathogenic of 484 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.72
gnomAD pLI
0
gnomAD missense Z
0.25
DepMap mean gene effect
-0.07
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 15% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DNAJC21 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DNAJC21 as an antibody target. Whether an autoantibody or antibody against DNAJC21 could matter depends on whether native DNAJC21 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DNAJC21 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DNAJC21 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/DNAJC21. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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