DNAJA3
DnaJ homolog subfamily A member 3, mitochondrial
Also known as: DNJA3_HUMAN, hTid-1, TID1
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q96EY1
- Gene
- DNAJA3
- Ensembl
- ENSG00000103423
- Chromosome
- 16
- Canonical length
- 480 aa
- Protein class
- Predicted intracellular proteins
- Subcellular location
- Vesicles,Mitochondria
OverviewNCBI Gene
This gene encodes a member of the DNAJ/Hsp40 protein family. DNAJ/Hsp40 proteins stimulate the ATPase activity of Hsp70 chaperones and play critical roles in protein folding, degradation, and multimeric complex assembly. The encoded protein is localized to mitochondria and mediates several cellular processes including proliferation, survival and apoptotic signal transduction. The encoded protein also plays a critical role in tumor suppression through interactions with oncogenic proteins including ErbB2 and the p53 tumor suppressor protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
Canonical amino-acid sequenceUniProt
480 residues, UniProt reviewed canonical sequence.
>Q96EY1|DNAJA3
1 MAARCSTRWL LVVVGTPRLP AISGRGARPP REGVVGAWLS RKLSVPAFAS SLTSCGPRAL
61 LTLRPGVSLT GTKHNPFICT ASFHTSAPLA KEDYYQILGV PRNASQKEIK KAYYQLAKKY
121 HPDTNKDDPK AKEKFSQLAE AYEVLSDEVK RKQYDAYGSA GFDPGASGSQ HSYWKGGPTV
181 DPEELFRKIF GEFSSSSFGD FQTVFDQPQE YFMELTFNQA AKGVNKEFTV NIMDTCERCN
241 GKGNEPGTKV QHCHYCGGSG METINTGPFV MRSTCRRCGG RGSIIISPCV VCRGAGQAKQ
301 KKRVMIPVPA GVEDGQTVRM PVGKREIFIT FRVQKSPVFR RDGADIHSDL FISIAQALLG
361 GTARAQGLYE TINVTIPPGT QTDQKIRMGG KGIPRINSYG YGDHYIHIKI RVPKRLTSRQ
421 QSLILSYAED ETDVEGTVNG VTLTSSGGST MDSSAGSKAR REAGEDEEGF LSKLKKMFTSLocalizationUniProt · AlphaFold · HPA
Whether an antibody against DNAJA3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Cell surface
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.47
- Highest tissue expression
- 87 nTPM
Expression across tissuesHPA
Tissue
- skeletal muscle: 87 nTPM
- liver: 63 nTPM
- tongue: 45 nTPM
- adrenal gland: 42 nTPM
- heart muscle: 42 nTPM
- esophagus: 27 nTPM
Single-cell type
- late primary spermatocytes: 43 nCPM
- early spermatids: 37 nCPM
- late spermatids: 33 nCPM
- renal collecting duct intercalated cells: 30 nCPM
- distal convoluted tubule cells: 28 nCPM
- other brain neurons: 28 nCPM
Immune cell
- naive CD4 T-cell: 13 nTPM
- MAIT T-cell: 12 nTPM
- memory B-cell: 12 nTPM
- NK-cell: 11 nTPM
- T-reg: 11 nTPM
- total PBMC: 11 nTPM
Brain region
- cerebellum: 19 nTPM
- thalamus: 19 nTPM
- pons: 18 nTPM
- cerebral cortex: 18 nTPM
- hypothalamus: 17 nTPM
- white matter: 17 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.59
- gnomAD pLI
- 0.01
- gnomAD missense Z
- -0.39
- DepMap mean gene effect
- -0.92
- DepMap dependency class
- common
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- activation-induced cell death of T cells
- cellular senescence
- mitochondrial DNA replication
- mitochondrion organization
- negative regulation of apoptotic process
- negative regulation of canonical NF-kappaB signal transduction
- negative regulation of cell population proliferation
- negative regulation of programmed cell death
- negative regulation of transcription by RNA polymerase II
- negative regulation of type II interferon-mediated signaling pathway
- neuromuscular junction development
- positive regulation of apoptotic process
- positive regulation of protein ubiquitination
- positive regulation of T cell proliferation
- protein folding
- protein stabilization
- response to heat
- response to type II interferon
- skeletal muscle acetylcholine-gated channel clustering
- small GTPase-mediated signal transduction
- T cell differentiation in thymus
Molecular functions
- ATP binding
- DNA-binding transcription factor binding
- GTPase regulator activity
- Hsp70 protein binding
- IkappaB kinase complex binding
- NF-kappaB binding
- protein kinase binding
- protein-containing complex binding
- RNA polymerase II-specific DNA-binding transcription factor binding
- signaling receptor binding
- type II interferon receptor binding
- unfolded protein binding
- zinc ion binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
- Heat shock protein DnaJ, cysteine-rich domain
- DnaJ domain
- Chaperone DnaJ, C-terminal
- HSP40/DnaJ peptide-binding
- Chaperone DnaJ
- DnaJ domain, conserved site
- Heat shock protein DnaJ, cysteine-rich domain superfamily
- Chaperone J-domain superfamily
- DnaJ domain
- DnaJ central domain
- DnaJ C terminal domain
- Apoptosis and cytoskeleton modulation protein
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of DNAJA3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads DNAJA3 as an antibody target. Whether an autoantibody or antibody against DNAJA3 could matter depends on whether native DNAJA3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
DNAJA3 is annotated at the cell surface, where native DNAJA3 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.
Annotation status
The present source text does not explicitly label DNAJA3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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