DGKI

Protein identityUniProt · HPA

UniProt accession

O75912

Gene

DGKI

Ensembl

ENSG00000157680

Chromosome

7

Canonical length

1057 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

OverviewNCBI Gene

This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1057 residues, UniProt reviewed canonical sequence.

>O75912|DGKI
     1  MDAAGRGCHL LPLPAARGPA RAPAAAAAAA ASPPGPCSGA ACAPSAAAGA GAMNPSSSAG
    61  EEKGATGGSS SSGSGAGSCC LGAEGGADPR GAGSAAAAGA AALDEPAAAG QKEKDEALEE
   121  KLRNLTFRKQ VSYRKAISRA GLQHLAPAHP LSLPVANGPA KEPRATLDWS ENAVNGEHLW
   181  LETNVSGDLC YLGEENCQVR FAKSALRRKC AVCKIVVHTA CIEQLEKINF RCKPTFREGG
   241  SRSPRENFVR HHWVHRRRQE GKCKQCGKGF QQKFSFHSKE IVAISCSWCK QAFHNKVTCF
   301  MLHHIEEPCS LGAHAAVIVP PTWIIKVKKP QNSLKASNRK KKRTSFKRKA SKRGMEQENK
   361  GRPFVIKPIS SPLMKPLLVF VNPKSGGNQG TKVLQMFMWY LNPRQVFDLS QEGPKDALEL
   421  YRKVPNLRIL ACGGDGTVGW ILSILDELQL SPQPPVGVLP LGTGNDLART LNWGGGYTDE
   481  PVSKILCQVE DGTVVQLDRW NLHVERNPDL PPEELEDGVC KLPLNVFNNY FSLGFDAHVT
   541  LEFHESREAN PEKFNSRFRN KMFYAGAAFS DFLQRSSRDL SKHVKVVCDG TDLTPKIQEL
   601  KFQCIVFLNI PRYCAGTMPW GNPGDHHDFE PQRHDDGYIE VIGFTMASLA ALQVGGHGER
   661  LHQCREVMLL TYKSIPMQVD GEPCRLAPAM IRISLRNQAN MVQKSKRRTS MPLLNDPQSV
   721  PDRLRIRVNK ISLQDYEGFH YDKEKLREAS IPLGILVVRG DCDLETCRMY IDRLQEDLQS
   781  VSSGSQRVHY QDHETSFPRA LSAQRLSPRW CFLDATSADR FYRIDRSQEH LHFVMEISQD
   841  EIFILDPDMV VSQPAGTPPG MPDLVVEQAS GISDWWNPAL RKRMLSDSGL GMIAPYYEDS
   901  DLKDLSHSRV LQSPVSSEDH AILQAVIAGD LMKLIESYKN GGSLLIQGPD HCSLLHYAAK
   961  TGNGEIVKYI LDHGPSELLD MADSETGETA LHKAACQRNR AVCQLLVDAG ASLRKTDSKG
  1021  KTPQERAQQA GDPDLAAYLE SRQNYKVIGH EDLETAV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DGKI can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.37

Highest tissue expression

15 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.31

gnomAD pLI

0.93

gnomAD missense Z

3.08

DepMap mean gene effect

0.03

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• diacylglycerol metabolic process
• intracellular signal transduction
• lipid phosphorylation
• phosphatidic acid biosynthetic process
• phospholipase C-activating G protein-coupled receptor signaling pathway
• platelet activation

Molecular functions

• ATP binding
• ATP-dependent diacylglycerol kinase activity

Cellular components

• axon
• cytoplasm
• cytosol
• dendritic spine
• glutamatergic synapse
• nucleus
• plasma membrane
• postsynaptic density
• protein-containing complex
• synapse
• synaptic membrane
• synaptic vesicle membrane

Protein domainsUniProt · Pfam · InterPro

• Diacylglycerol kinase, accessory domain
• Diacylglycerol kinase, catalytic domain
• Ankyrin repeat
• Protein kinase C-like, phorbol ester/diacylglycerol-binding domain
• NAD kinase/diacylglycerol kinase-like domain superfamily
• Inorganic polyphosphate/ATP-NAD kinase, N-terminal
• Ankyrin repeat-containing domain superfamily
• Diacylglycerol kinase
• Diacylglycerol kinase iota-like domain
• Phorbol esters/diacylglycerol binding domain (C1 domain)
• Diacylglycerol kinase accessory domain
• Diacylglycerol kinase catalytic domain
• Ankyrin repeats (3 copies)
• Diacylglycerol kinase iota-like domain
• Diacylglycerol kinase iota, first protein kinase C conserved region 1
• Diacylglycerol kinase iota, second protein kinase C conserved region 1

KeywordsUniProt

• ANK repeat
• ATP-binding
• Cell membrane
• Cell projection
• Cytoplasm
• Cytoplasmic vesicle
• Kinase
• Lipid metabolism
• Membrane
• Nucleotide-binding
• Nucleus
• Repeat
• Synapse
• Transferase

InteractionsUniProt · HPA

Protein binding partners of DGKI in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• DLG2
• DLG4
• DLG3
• DLG1