Seroatlas · Human Serome Atlas

DENND1A

DENN domain-containing protein 1A

Also known as: DEN1A_HUMAN, FAM31A, FLJ21129, KIAA1608

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8TEH3
Gene: DENND1A
Ensembl: ENSG00000119522
Chromosome: 9
Canonical length: 1009 aa
Protein class: Disease related genes, Plasma proteins, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location: Vesicles,Cytosol

OverviewNCBI Gene

Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1A, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).[supplied by OMIM, Nov 2010]

Canonical amino-acid sequenceUniProt

1009 residues, UniProt reviewed canonical sequence.

>Q8TEH3|DENND1A
     1  MGSRIKQNPE TTFEVYVEVA YPRTGGTLSD PEVQRQFPED YSDQEVLQTL TKFCFPFYVD
    61  SLTVSQVGQN FTFVLTDIDS KQRFGFCRLS SGAKSCFCIL SYLPWFEVFY KLLNILADYT
   121  TKRQENQWNE LLETLHKLPI PDPGVSVHLS VHSYFTVPDT RELPSIPENR NLTEYFVAVD
   181  VNNMLHLYAS MLYERRILII CSKLSTLTAC IHGSAAMLYP MYWQHVYIPV LPPHLLDYCC
   241  APMPYLIGIH LSLMEKVRNM ALDDVVILNV DTNTLETPFD DLQSLPNDVI SSLKNRLKKV
   301  STTTGDGVAR AFLKAQAAFF GSYRNALKIE PEEPITFCEE AFVSHYRSGA MRQFLQNATQ
   361  LQLFKQFIDG RLDLLNSGEG FSDVFEEEIN MGEYAGSDKL YHQWLSTVRK GSGAILNTVK
   421  TKANPAMKTV YKFAKDHAKM GIKEVKNRLK QKDIAENGCA PTPEEQLPKT APSPLVEAKD
   481  PKLREDRRPI TVHFGQVRPP RPHVVKRPKS NIAVEGRRTS VPSPEQPQPY RTLRESDSAE
   541  GDEAESPEQQ VRKSTGPVPA PPDRAASIDL LEDVFSNLDM EAALQPLGQA KSLEDLRAPK
   601  DLREQPGTFD YQRLDLGGSE RSRGVTVALK LTHPYNKLWS LGQDDMAIPS KPPAASPEKP
   661  SALLGNSLAL PRRPQNRDSI LNPSDKEEVP TPTLGSITIP RPQGRKTPEL GIVPPPPIPR
   721  PAKLQAAGAA LGDVSERLQT DRDRRAALSP GLLPGVVPQG PTELLQPLSP GPGAAGTSSD
   781  ALLALLDPLS TAWSGSTLPS RPATPNVATP FTPQFSFPPA GTPTPFPQPP LNPFVPSMPA
   841  APPTLPLVST PAGPFGAPPA SLGPAFASGL LLSSAGFCAP HRSQPNLSAL SMPNLFGQMP
   901  MGTHTSPLQP LGPPAVAPSR IRTLPLARSS ARAAETKQGL ALRPGDPPLL PPRPPQGLEP
   961  TLQPSAPQQA RDPFEDLLQK TKQDVSPSPA LAPAPDSVEQ LRKQWETFE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DENND1A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.52
Highest tissue expression: 19 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 19 nTPM
cerebral cortex: 15 nTPM
spleen: 13 nTPM
amygdala: 11 nTPM
kidney: 11 nTPM
skeletal muscle: 11 nTPM

Single-cell type

neutrophils: 676 nCPM
pituicytes/fscs: 548 nCPM
neutrophil progenitors: 537 nCPM
monocyte progenitors: 507 nCPM
choroid plexus epithelial cells: 502 nCPM
monocytes: 501 nCPM

Immune cell

classical monocyte: 20 nTPM
basophil: 14 nTPM
neutrophil: 13 nTPM
intermediate monocyte: 12 nTPM
myeloid DC: 11 nTPM
non-classical monocyte: 9.1 nTPM

Brain region

cerebral cortex: 38 nTPM
basal ganglia: 37 nTPM
amygdala: 36 nTPM
hippocampal formation: 36 nTPM
midbrain: 32 nTPM
hypothalamus: 32 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.23
gnomAD pLI: 1
gnomAD missense Z: 1.26
DepMap mean gene effect: -0.05
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of DENND1A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DENND1A as an antibody target. Whether an autoantibody or antibody against DENND1A could matter depends on whether native DENND1A is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DENND1A is annotated at the cell surface, where native DENND1A is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label DENND1A as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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