DDX53

Protein identityUniProt · HPA

UniProt accession

Q86TM3

Gene

DDX53

Ensembl

ENSG00000184735

Chromosome

X

Canonical length

631 aa

Protein class

Enzymes, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Nucleoli,Cytosol

OverviewNCBI Gene

This intronless gene encodes a protein which contains several domains found in members of the DEAD-box helicase protein family. Other members of this protein family participate in ATP-dependent RNA unwinding. [provided by RefSeq, Sep 2011]

Canonical amino-acid sequenceUniProt

631 residues, UniProt reviewed canonical sequence.

>Q86TM3|DDX53
     1  MSHWAPEWKR AEANPRDLGA SWDVRGSRGS GWSGPFGHQG PRAAGSREPP LCFKIKNNMV
    61  GVVIGYSGSK IKDLQHSTNT KIQIINGESE AKVRIFGNRE MKAKAKAAIE TLIRKQESYN
   121  SESSVDNAAS QTPIGRNLGR NDIVGEAEPL SNWDRIRAAV VECEKRKWAD LPPVKKNFYI
   181  ESKATSCMSE MQVINWRKEN FNITCDDLKS GEKRLIPKPT CRFKDAFQQY PDLLKSIIRV
   241  GIVKPTPIQS QAWPIILQGI DLIVVAQTGT GKTLSYLMPG FIHLDSQPIS REQRNGPGML
   301  VLTPTRELAL HVEAECSKYS YKGLKSICIY GGRNRNGQIE DISKGVDIII ATPGRLNDLQ
   361  MNNSVNLRSI TYLVIDEADK MLDMEFEPQI RKILLDVRPD RQTVMTSATW PDTVRQLALS
   421  YLKDPMIVYV GNLNLVAVNT VKQNIIVTTE KEKRALTQEF VENMSPNDKV IMFVSQKHIA
   481  DDLSSDFNIQ GISAESLHGN SEQSDQERAV EDFKSGNIKI LITTDIVSRG LDLNDVTHVY
   541  NYDFPRNIDV YVHRVGYIGR TGKTGTSVTL ITQRDSKMAG ELIKILDRAN QSVPEDLVVM
   601  AEQYKLNQQK RHRETRSRKP GQRRKEFYFL S

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DDX53 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.34

Highest tissue expression

5 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for DDX53 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.85

gnomAD pLI

0.66

gnomAD missense Z

0.01

DepMap mean gene effect

0.07

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

• ATP binding
• ATP hydrolysis activity
• mRNA binding
• RNA helicase activity

Cellular components

• cytosol
• nucleolus
• nucleoplasm

Protein domainsUniProt · Pfam · InterPro

• ATP-dependent RNA helicase DEAD-box, conserved site
• Helicase, C-terminal domain-like
• K Homology domain
• K Homology domain, type 1
• DEAD/DEAH-box helicase domain
• Helicase superfamily 1/2, ATP-binding domain
• P-loop containing nucleoside triphosphate hydrolase
• K Homology domain, type 1 superfamily
• KH domain
• DEAD/DEAH box helicase
• Helicase conserved C-terminal domain

KeywordsUniProt

• ATP-binding
• Helicase
• Hydrolase
• Nucleotide-binding
• Nucleus
• RNA-binding