DDX46

Protein identityUniProt · HPA

UniProt accession

Q7L014

Gene

DDX46

Ensembl

ENSG00000145833

Chromosome

5

Canonical length

1031 aa

Protein class

Enzymes, Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoli fibrillar center,Nuclear speckles

OverviewNCBI Gene

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the 17S U2 snRNP complex; it plays an important role in pre-mRNA splicing. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2014]

Canonical amino-acid sequenceUniProt

1031 residues, UniProt reviewed canonical sequence.

>Q7L014|DDX46
     1  MGRESRHYRK RSASRGRSGS RSRSRSPSDK RSKRGDDRRS RSRDRDRRRE RSRSRDKRRS
    61  RSRDRKRLRR SRSRERDRSR ERRRSRSRDR RRSRSRSRGR RSRSSSPGNK SKKTENRSRS
   121  KEKTDGGESS KEKKKDKDDK EDEKEKDAGN FDQNKLEEEM RKRKERVEKW REEQRKKAME
   181  NIGELKKEIE EMKQGKKWSL EDDDDDEDDP AEAEKEGNEM EGEELDPLDA YMEEVKEEVK
   241  KFNMRSVKGG GGNEKKSGPT VTKVVTVVTT KKAVVDSDKK KGELMENDQD AMEYSSEEEE
   301  VDLQTALTGY QTKQRKLLEP VDHGKIEYEP FRKNFYVEVP ELAKMSQEEV NVFRLEMEGI
   361  TVKGKGCPKP IKSWVQCGIS MKILNSLKKH GYEKPTPIQT QAIPAIMSGR DLIGIAKTGS
   421  GKTIAFLLPM FRHIMDQRSL EEGEGPIAVI MTPTRELALQ ITKECKKFSK TLGLRVVCVY
   481  GGTGISEQIA ELKRGAEIIV CTPGRMIDML AANSGRVTNL RRVTYVVLDE ADRMFDMGFE
   541  PQVMRIVDNV RPDRQTVMFS ATFPRAMEAL ARRILSKPIE VQVGGRSVVC SDVEQQVIVI
   601  EEEKKFLKLL ELLGHYQESG SVIIFVDKQE HADGLLKDLM RASYPCMSLH GGIDQYDRDS
   661  IINDFKNGTC KLLVATSVAA RGLDVKHLIL VVNYSCPNHY EDYVHRAGRT GRAGNKGYAY
   721  TFITEDQARY AGDIIKALEL SGTAVPPDLE KLWSDFKDQQ KAEGKIIKKS SGFSGKGFKF
   781  DETEQALANE RKKLQKAALG LQDSDDEDAA VDIDEQIESM FNSKKRVKDM AAPGTSSVPA
   841  PTAGNAEKLE IAKRLALRIN AQKNLGIESQ DVMQQATNAI LRGGTILAPT VSAKTIAEQL
   901  AEKINAKLNY VPLEKQEEER QDGGQNESFK RYEEELEIND FPQTARWKVT SKEALQRISE
   961  YSEAAITIRG TYFPPGKEPK EGERKIYLAI ESANELAVQK AKAEITRLIK EELIRLQNSY
  1021  QPTNKGRYKV L

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DDX46 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.43

Highest tissue expression

33 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.14

gnomAD pLI

1

gnomAD missense Z

5.48

DepMap mean gene effect

-1.02

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• mRNA splicing, via spliceosome
• U2-type prespliceosome assembly

Molecular functions

• ATP binding
• ATP hydrolysis activity
• RNA binding
• RNA helicase activity

Cellular components

• Cajal body
• fibrillar center
• nuclear speck
• nucleoplasm
• nucleus
• spliceosomal complex
• U2 snRNP
• U2-type spliceosomal complex

Protein domainsUniProt · Pfam · InterPro

• ATP-dependent RNA helicase DEAD-box, conserved site
• Helicase, C-terminal domain-like
• DEAD/DEAH-box helicase domain
• Helicase superfamily 1/2, ATP-binding domain
• RNA helicase, DEAD-box type, Q motif
• P-loop containing nucleoside triphosphate hydrolase
• ATP-dependent RNA helicase PRP5/DDX46/KHDC4, KH domain
• DEAD/DEAH box helicase
• Helicase conserved C-terminal domain
• KH-domain

KeywordsUniProt

• Acetylation
• ATP-binding
• Coiled coil
• Helicase
• Hydrolase
• Isopeptide bond
• Lipoprotein
• mRNA processing
• mRNA splicing
• Myristate
• Nucleotide-binding
• Nucleus
• Phosphoprotein
• RNA-binding
• Spliceosome
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of DDX46 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• SNRPA1
• SRPK2