CDH5

Protein identityUniProt · HPA

UniProt accession

P33151

Gene

CDH5

Ensembl

ENSG00000179776

Chromosome

16

Canonical length

784 aa

Protein class

Cancer-related genes, CD markers, Plasma proteins, Predicted intracellular proteins, Predicted membrane proteins

Subcellular location

Nucleoplasm,Nuclear membrane,Plasma membrane

OverviewNCBI Gene

This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. [provided by RefSeq, Nov 2015]

Canonical amino-acid sequenceUniProt

784 residues, UniProt reviewed canonical sequence.

>P33151|CDH5
     1  MQRLMMLLAT SGACLGLLAV AAVAAAGANP AQRDTHSLLP THRRQKRDWI WNQMHIDEEK
    61  NTSLPHHVGK IKSSVSRKNA KYLLKGEYVG KVFRVDAETG DVFAIERLDR ENISEYHLTA
   121  VIVDKDTGEN LETPSSFTIK VHDVNDNWPV FTHRLFNASV PESSAVGTSV ISVTAVDADD
   181  PTVGDHASVM YQILKGKEYF AIDNSGRIIT ITKSLDREKQ ARYEIVVEAR DAQGLRGDSG
   241  TATVLVTLQD INDNFPFFTQ TKYTFVVPED TRVGTSVGSL FVEDPDEPQN RMTKYSILRG
   301  DYQDAFTIET NPAHNEGIIK PMKPLDYEYI QQYSFIVEAT DPTIDLRYMS PPAGNRAQVI
   361  INITDVDEPP IFQQPFYHFQ LKENQKKPLI GTVLAMDPDA ARHSIGYSIR RTSDKGQFFR
   421  VTKKGDIYNE KELDREVYPW YNLTVEAKEL DSTGTPTGKE SIVQVHIEVL DENDNAPEFA
   481  KPYQPKVCEN AVHGQLVLQI SAIDKDITPR NVKFKFILNT ENNFTLTDNH DNTANITVKY
   541  GQFDREHTKV HFLPVVISDN GMPSRTGTST LTVAVCKCNE QGEFTFCEDM AAQVGVSIQA
   601  VVAILLCILT ITVITLLIFL RRRLRKQARA HGKSVPEIHE QLVTYDEEGG GEMDTTSYDV
   661  SVLNSVRRGG AKPPRPALDA RPSLYAQVQK PPRHAPGAHG GPGEMAAMIE VKKDEADHDG
   721  DGPPYDTLHI YGYEGSESIA ESLSSLGTDS SDSDVDYDFL NDWGPRFKML AELYGSDPRE
   781  ELLY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CDH5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.41

Highest tissue expression

222 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for CDH5 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.4

gnomAD pLI

0.51

gnomAD missense Z

0.52

DepMap mean gene effect

-0.09

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• adherens junction organization
• bicellular tight junction assembly
• blood vessel endothelial cell migration
• blood vessel maturation
• calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules
• cell migration
• cell migration involved in sprouting angiogenesis
• cell morphogenesis
• cell surface receptor signaling pathway
• cell-cell adhesion
• cell-cell adhesion mediated by cadherin
• cell-cell junction assembly
• endothelial cell morphogenesis
• homophilic cell adhesion via plasma membrane adhesion molecules
• intracellular calcium ion homeostasis
• maintenance of blood-brain barrier
• negative regulation of cell population proliferation
• negative regulation of endothelial cell apoptotic process
• negative regulation of inflammatory response
• negative regulation of microtubule polymerization
• positive regulation of angiogenesis
• positive regulation of BMP signaling pathway
• positive regulation of cell migration
• positive regulation of establishment of endothelial barrier
• positive regulation of gene expression
• positive regulation of intracellular signal transduction
• positive regulation of protein-containing complex assembly
• protein localization to bicellular tight junction
• regulation of establishment of cell polarity
• regulation of vascular permeability
• transforming growth factor beta receptor signaling pathway

Molecular functions

• beta-catenin binding
• BMP receptor binding
• cadherin binding
• calcium ion binding
• cell-cell adhesion mediator activity
• fibrinogen binding
• protein phosphatase binding
• protein tyrosine kinase binding
• signaling receptor binding
• signaling receptor complex adaptor activity
• transmembrane transporter binding
• vascular endothelial growth factor receptor 2 binding

Cellular components

• adherens junction
• bicellular tight junction
• catenin complex
• cell junction
• cell surface
• cell-cell junction
• cytoplasm
• external side of plasma membrane
• membrane
• nuclear membrane
• nucleoplasm
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Cadherin, Y-type LIR-motif
• Cadherin-like
• Cadherin-like superfamily
• Cadherin conserved site
• Catenin binding domain superfamily
• Cadherin
• Cadherin domain
• Cadherin, Y-type LIR-motif

KeywordsUniProt

• Calcium
• Cell adhesion
• Cell junction
• Cell membrane
• Cleavage on pair of basic residues
• Cytoplasm
• Glycoprotein
• Membrane
• Metal-binding
• Phosphoprotein
• Repeat
• Signal
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of CDH5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• KRIT1
• PALS1
• PARD3
• RTN4
• TRPC4
• AMOTL2
• PTPRB
• CTNNB1
• KDR