Seroatlas · Human Serome Atlas

CDCA4

Cell division cycle-associated protein 4

Also known as: CDCA4_HUMAN, FLJ20764, Hepp

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9BXL8
Gene
CDCA4
Ensembl
ENSG00000170779
Chromosome
14
Canonical length
241 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Plasma membrane,Cytosol

OverviewNCBI Gene

This gene encodes a protein that belongs to the E2F family of transcription factors. This protein regulates E2F-dependent transcriptional activation and cell proliferation, mainly through the E2F/retinoblastoma protein pathway. It also functions in the regulation of JUN oncogene expression. This protein shows distinctive nuclear-mitotic apparatus distribution, it is involved in spindle organization from prometaphase, and may also play a role as a midzone factor involved in chromosome segregation or cytokinesis. Two alternatively spliced transcript variants encoding the same protein have been noted for this gene. Two pseudogenes have also been identified on chromosome 1. [provided by RefSeq, May 2014]

Canonical amino-acid sequenceUniProt

241 residues, UniProt reviewed canonical sequence.

>Q9BXL8|CDCA4
     1  MFARGLKRKC VGHEEDVEGA LAGLKTVSSY SLQRQSLLDM SLVKLQLCHM LVEPNLCRSV
    61  LIANTVRQIQ EEMTQDGTWR TVAPQAAERA PLDRLVSTEI LCRAAWGQEG AHPAPGLGDG
   121  HTQGPVSDLC PVTSAQAPRH LQSSAWEMDG PRENRGSFHK SLDQIFETLE TKNPSCMEEL
   181  FSDVDSPYYD LDTVLTGMMG GARPGPCEGL EGLAPATPGP SSSCKSDLGE LDHVVEILVE
   241  T

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CDCA4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.59
Highest tissue expression
53 nTPM

Expression across tissuesHPA

Tissue

  • bone marrow: 53 nTPM
  • skin: 24 nTPM
  • esophagus: 18 nTPM
  • thymus: 14 nTPM
  • testis: 11 nTPM
  • lymph node: 11 nTPM

Single-cell type

  • extravillous trophoblasts: 192 nCPM
  • migrating cytotrophoblasts: 139 nCPM
  • cytotrophoblasts: 96 nCPM
  • erythrocyte progenitors: 95 nCPM
  • esophageal basal cells: 94 nCPM
  • differentiating spermatogonia: 90 nCPM

Immune cell

  • memory CD8 T-cell: 17 nTPM
  • MAIT T-cell: 15 nTPM
  • NK-cell: 13 nTPM
  • memory CD4 T-cell: 13 nTPM
  • gdT-cell: 12 nTPM
  • T-reg: 11 nTPM

Brain region

  • cerebellum: 4.9 nTPM
  • medulla oblongata: 3.1 nTPM
  • choroid plexus: 3 nTPM
  • pons: 2.9 nTPM
  • thalamus: 2.8 nTPM
  • basal ganglia: 2.7 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.38
gnomAD pLI
0.08
gnomAD missense Z
0.3
DepMap mean gene effect
0.09
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CDCA4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CDCA4 as an antibody target. Whether an autoantibody or antibody against CDCA4 could matter depends on whether native CDCA4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CDCA4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CDCA4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CDCA4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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