BCAR3

Protein identityUniProt · HPA

UniProt accession

O75815

Gene

BCAR3

Ensembl

ENSG00000137936

Chromosome

1

Canonical length

825 aa

Protein class

Predicted intracellular proteins

Subcellular location

Plasma membrane,Centrosome,Cytosol

OverviewNCBI Gene

Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Canonical amino-acid sequenceUniProt

825 residues, UniProt reviewed canonical sequence.

>O75815|BCAR3
     1  MAAGKFASLP RNMPVNHQFP LASSMDLLSS RSPLAEHRPD AYQDVSIHGT LPRKKKGPPP
    61  IRSCDDFSHM GTLPHSKSPR QNSPVTQDGI QESPWQDRHG ETFTFRDPHL LDPTVEYVKF
   121  SKERHIMDRT PEKLKKELEE ELLLSSEDLR SHAWYHGRIP RQVSENLVQR DGDFLVRDSL
   181  SSPGNFVLTC QWKNLAQHFK INRTVLRLSE AYSRVQYQFE MESFDSIPGL VRCYVGNRRP
   241  ISQQSGAIIF QPINRTVPLR CLEEHYGTSP GQAREGSLTK GRPDVAKRLS LTMGGVQARE
   301  QNLPRGNLLR NKEKSGSQPA CLDHMQDRRA LSLKAHQSES YLPIGCKLPP QSSGVDTSPC
   361  PNSPVFRTGS EPALSPAVVR RVSSDARAGE ALRGSDSQLC PKPPPKPCKV PFLKVPSSPS
   421  AWLNSEANYC ELNPAFATGC GRGAKLPSCA QGSHTELLTA KQNEAPGPRN SGVNYLILDD
   481  DDRERPWEPA AAQMEKGQWD KGEFVTPLLE TVSSFRPNEF ESKFLPPENK PLETAMLKRA
   541  KELFTNNDPK VIAQHVLSMD CRVARILGVS EEMRRNMGVS SGLELITLPH GHQLRLDIIE
   601  RHNTMAIGIA VDILGCTGTL EDRAATLSKI IQVAVELKDS MGDLYSFSAL MKALEMPQIT
   661  RLEKTWTALR HQYTQTAILY EKQLKPFSKL LHEGRESTCV PPNNVSVPLL MPLVTLMERQ
   721  AVTFEGTDMW EKNDQSCEIM LNHLATARFM AEAADSYRMN AERILAGFQP DEEMNEICKT
   781  EFQMRLLWGS KGAQVNQTER YEKFNQILTA LSRKLEPPPV KQAEL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against BCAR3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.47

Highest tissue expression

29 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about BCAR3.

Disease | ImmuneIEDB

Conditions an epitope on BCAR3 was assayed in.

• skin melanoma T cell
• melanoma T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.38

gnomAD pLI

0.58

gnomAD missense Z

0.3

DepMap mean gene effect

-0.11

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• endothelin receptor signaling pathway
• epidermal growth factor receptor signaling pathway
• insulin receptor signaling pathway
• lens morphogenesis in camera-type eye
• positive regulation of cell population proliferation
• positive regulation of GTPase activity
• positive regulation of MAPK cascade
• response to xenobiotic stimulus
• signal transduction
• small GTPase-mediated signal transduction

Molecular functions

• guanyl-nucleotide exchange factor activity
• kinase binding
• phosphotyrosine residue binding

Cellular components

• cytoplasm
• focal adhesion
• membrane

Protein domainsUniProt · Pfam · InterPro

• SH2 domain
• Ras guanine-nucleotide exchange factors catalytic domain
• Ras guanine nucleotide exchange factor domain superfamily
• SH2 domain superfamily
• Ras guanine-nucleotide exchange factor, catalytic domain superfamily
• SHEP1/BCAR3/NSP1, SH2 domain
• Adapter protein with SH2 and Ras-GEF domains
• SH2 domain
• RasGEF domain

KeywordsUniProt

• Acetylation
• Cell junction
• Cytoplasm
• Guanine-nucleotide releasing factor
• Methylation
• Phosphoprotein
• SH2 domain

InteractionsUniProt · HPA

Protein binding partners of BCAR3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• PTPN1
• PTPN11
• BCAR1
• NEDD9
• IRS1
• OSGIN1