ARF3

Protein identityUniProt · HPA

UniProt accession

P61204

Gene

ARF3

Ensembl

ENSG00000134287

Chromosome

12

Canonical length

181 aa

Protein class

Plasma proteins, Predicted intracellular proteins

OverviewNCBI Gene

ADP-ribosylation factor 3 (ARF3) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6) and members of each class share a common gene organization. [provided by RefSeq, Oct 2022]

Canonical amino-acid sequenceUniProt

181 residues, UniProt reviewed canonical sequence.

>P61204|ARF3
     1  MGNIFGNLLK SLIGKKEMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN
    61  ISFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV NEAREELMRM LAEDELRDAV
   121  LLVFANKQDL PNAMNAAEIT DKLGLHSLRH RNWYIQATCA TSGDGLYEGL DWLANQLKNK
   181  K

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ARF3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.28

Highest tissue expression

271 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ARF3.

Disease | GeneticClinVar

10 pathogenic / likely-pathogenic of 41 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Intellectual disability
• Atrophy/Degeneration affecting the central nervous system
• ARF3-related disorder
• Microcephaly
• Scoliosis

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.72

gnomAD pLI

0.35

gnomAD missense Z

2.82

DepMap mean gene effect

-0.23

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• intracellular protein transport
• retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum
• vesicle-mediated transport

Molecular functions

• GTP binding
• GTPase activity

Cellular components

• cytoplasm
• extracellular exosome
• Golgi membrane
• perinuclear region of cytoplasm
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Small GTP-binding domain
• Small GTPase superfamily, ARF/SAR type
• Small GTPase superfamily, ARF type
• P-loop containing nucleoside triphosphate hydrolase
• ADP-ribosylation factor 1-5
• ADP-ribosylation factor family

KeywordsUniProt

• Cytoplasm
• ER-Golgi transport
• Golgi apparatus
• GTP-binding
• Lipoprotein
• Myristate
• Nucleotide-binding
• Protein transport
• Transport

InteractionsUniProt · HPA

Protein binding partners of ARF3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• PI4KB
• PRKCABP
• GBF1
• NCS1
• ARF1