Seroatlas · Human Serome Atlas

ANKRD27

Ankyrin repeat domain-containing protein 27

Also known as: ANR27_HUMAN, DKFZp434L0718, FLJ00040, VARP

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q96NW4
Gene
ANKRD27
Ensembl
ENSG00000105186
Chromosome
19
Canonical length
1050 aa
Protein class
Predicted intracellular proteins
Subcellular location
Vesicles,Cytosol
Quaternary structure
Homodimer

OverviewNCBI Gene

Enables guanyl-nucleotide exchange factor activity and small GTPase binding activity. Involved in endocytic recycling and negative regulation of SNARE complex assembly. Acts upstream of or within early endosome to late endosome transport. Located in several cellular components, including cytosol; endosome; and lysosome. Implicated in eosinophilic esophagitis. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1050 residues, UniProt reviewed canonical sequence.

>Q96NW4|ANKRD27
     1  MALYDEDLLK NPFYLALQKC RPDLCSKVAQ IHGIVLVPCK GSLSSSIQST CQFESYILIP
    61  VEEHFQTLNG KDVFIQGNRI KLGAGFACLL SVPILFEETF YNEKEESFSI LCIAHPLEKR
   121  ESSEEPLAPS DPFSLKTIED VREFLGRHSE RFDRNIASFH RTFRECERKS LRHHIDSANA
   181  LYTKCLQQLL RDSHLKMLAK QEAQMNLMKQ AVEIYVHHEI YNLIFKYVGT MEASEDAAFN
   241  KITRSLQDLQ QKDIGVKPEF SFNIPRAKRE LAQLNKCTSP QQKLVCLRKV VQLITQSPSQ
   301  RVNLETMCAD DLLSVLLYLL VKTEIPNWMA NLSYIKNFRF SSLAKDELGY CLTSFEAAIE
   361  YIRQGSLSAK PPESEGFGDR LFLKQRMSLL SQMTSSPTDC LFKHIASGNQ KEVERLLSQE
   421  DHDKDTVQKM CHPLCFCDDC EKLVSGRLND PSVVTPFSRD DRGHTPLHVA AVCGQASLID
   481  LLVSKGAMVN ATDYHGATPL HLACQKGYQS VTLLLLHYKA SAEVQDNNGN TPLHLACTYG
   541  HEDCVKALVY YDVESCRLDI GNEKGDTPLH IAARWGYQGV IETLLQNGAS TEIQNRLKET
   601  PLKCALNSKI LSVMEAYHLS FERRQKSSEA PVQSPQRSVD SISQESSTSS FSSMSASSRQ
   661  EETKKDYREV EKLLRAVADG DLEMVRYLLE WTEEDLEDAE DTVSAADPEF CHPLCQCPKC
   721  APAQKRLAKV PASGLGVNVT SQDGSSPLHV AALHGRADLI PLLLKHGANA GARNADQAVP
   781  LHLACQQGHF QVVKCLLDSN AKPNKKDLSG NTPLIYACSG GHHELVALLL QHGASINASN
   841  NKGNTALHEA VIEKHVFVVE LLLLHGASVQ VLNKRQRTAV DCAEQNSKIM ELLQVVPSCV
   901  ASLDDVAETD RKEYVTVKIR KKWNSKLYDL PDEPFTRQFY FVHSAGQFKG KTSREIMARD
   961  RSVPNLTEGS LHEPGRQSVT LRQNNLPAQS GSHAAEKGNS DWPERPGLTQ TGPGHRRMLR
  1021  RHTVEDAVVS QGPEAAGPLS TPQEVSASRS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ANKRD27 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.37
Highest tissue expression
20 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 20 nTPM
  • choroid plexus: 19 nTPM
  • urinary bladder: 17 nTPM
  • colon: 16 nTPM
  • testis: 16 nTPM
  • thymus: 16 nTPM

Single-cell type

  • cone photoreceptor cells: 193 nCPM
  • cardiomyocytes: 168 nCPM
  • megakaryocyte-erythroid progenitors: 142 nCPM
  • myonuclei: 118 nCPM
  • rod photoreceptor cells: 104 nCPM
  • retinal ganglion cells: 100 nCPM

Immune cell

  • basophil: 37 nTPM
  • NK-cell: 31 nTPM
  • eosinophil: 29 nTPM
  • MAIT T-cell: 18 nTPM
  • intermediate monocyte: 16 nTPM
  • memory CD8 T-cell: 16 nTPM

Brain region

  • choroid plexus: 49 nTPM
  • midbrain: 32 nTPM
  • cerebral cortex: 30 nTPM
  • pons: 30 nTPM
  • thalamus: 29 nTPM
  • medulla oblongata: 29 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.11
gnomAD pLI
0
gnomAD missense Z
-0.27
DepMap mean gene effect
-0.11
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ANKRD27 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ANKRD27 as an antibody target. Whether an autoantibody or antibody against ANKRD27 could matter depends on whether native ANKRD27 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ANKRD27 is annotated at the cell surface, where native ANKRD27 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label ANKRD27 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ANKRD27. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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