AKAP5
A-kinase anchor protein 5
Also known as: AKAP5_HUMAN, AKAP75, AKAP79
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P24588
- Gene
- AKAP5
- Ensembl
- ENSG00000179841
- Chromosome
- 14
- Canonical length
- 427 aa
- Protein class
- Plasma proteins, Predicted intracellular proteins, Transporters
- Subcellular location
- Golgi apparatus,Plasma membrane,Cytosol
OverviewNCBI Gene
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT. [provided by RefSeq, Jul 2008]
Canonical amino-acid sequenceUniProt
427 residues, UniProt reviewed canonical sequence.
>P24588|AKAP5
1 METTISEIHV ENKDEKRSAE GSPGAERQKE KASMLCFKRR KKAAKALKPK AGSEAADVAR
61 KCPQEAGASD QPEPTRGAWA SLKRLVTRRK RSESSKQQKP LEGEMQPAIN AEDADLSKKK
121 AKSRLKIPCI KFPRGPKRSN HSKIIEDSDC SIKVQEEAEI LDIQTQTPLN DQATKAKSTQ
181 DLSEGISRKD GDEVCESNVS NSTTSGEKVI SVELGLDNGH SAIQTGTLIL EEIETIKEKQ
241 DVQPQQASPL ETSETDHQQP VLSDVPPLPA IPDQQIVEEA SNSTLESAPN GKDYESTEIV
301 AEETKPKDTE LSQESDFKEN GITEEKSKSE ESKRMEPIAI IITDTEISEF DVTKSKNVPK
361 QFLISAENEQ VGVFANDNGF EDRTSEQYET LLIETASSLV KNAIQLSIEQ LVNEMASDDN
421 KINNLLQLocalizationUniProt · AlphaFold · HPA
Whether an antibody against AKAP5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Cell surface
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.69
- Highest tissue expression
- 18 nTPM
Expression across tissuesHPA
Tissue
- basal ganglia: 18 nTPM
- cerebral cortex: 13 nTPM
- amygdala: 5.5 nTPM
- hippocampal formation: 4.6 nTPM
- rectum: 3.6 nTPM
- colon: 3.5 nTPM
Single-cell type
- mast cells: 39 nCPM
- goblet cells: 36 nCPM
- nk-cells: 30 nCPM
- brain inhibitory neurons: 29 nCPM
- cardiomyocytes: 28 nCPM
- brain excitatory neurons: 16 nCPM
Immune cell
- basophil: 2.9 nTPM
- gdT-cell: 0.3 nTPM
- memory CD8 T-cell: 0.3 nTPM
- naive B-cell: 0.2 nTPM
- NK-cell: 0.2 nTPM
- memory B-cell: 0.1 nTPM
Brain region
- basal ganglia: 40 nTPM
- cerebral cortex: 29 nTPM
- amygdala: 23 nTPM
- hippocampal formation: 22 nTPM
- white matter: 12 nTPM
- thalamus: 8.8 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.52
- gnomAD pLI
- 0.65
- gnomAD missense Z
- 1.17
- DepMap mean gene effect
- 0.01
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
- calcineurin-NFAT signaling cascade
- chemical synaptic transmission
- negative regulation of adenylate cyclase activity
- positive regulation of calcineurin-NFAT signaling cascade
- positive regulation of calcium ion import across plasma membrane
- positive regulation of endosome to plasma membrane protein transport
- positive regulation of long-term synaptic potentiation
- positive regulation of protein localization to plasma membrane
- regulation of protein kinase A signaling
- signal transduction
Molecular functions
- adenylate cyclase binding
- beta-2 adrenergic receptor binding
- calmodulin binding
- GABA receptor binding
- glutamate receptor binding
- molecular adaptor activity
- protein kinase A binding
- protein kinase A regulatory subunit binding
- protein phosphatase 2B binding
- scaffold protein binding
- SH3 domain binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
- A kinase-anchoring protein AKAP5 and AKAP12, calmodulin (CaM)-binding motif
- WSK motif
- A-kinase anchor protein 5
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of AKAP5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads AKAP5 as an antibody target. Whether an autoantibody or antibody against AKAP5 could matter depends on whether native AKAP5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
AKAP5 is annotated at the cell surface, where native AKAP5 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.
Annotation status
The present source text does not explicitly label AKAP5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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