ACSS1

Protein identityUniProt · HPA

UniProt accession

Q9NUB1

Gene

ACSS1

Ensembl

ENSG00000154930

Chromosome

20

Canonical length

689 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins

OverviewNCBI Gene

This gene encodes a mitochondrial acetyl-CoA synthetase enzyme. A similar protein in mice plays an important role in the tricarboxylic acid cycle by catalyzing the conversion of acetate to acetyl CoA. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Canonical amino-acid sequenceUniProt

689 residues, UniProt reviewed canonical sequence.

>Q9NUB1|ACSS1
     1  MAARTLGRGV GRLLGSLRGL SGQPARPPCG VSAPRRAASG PSGSAPAVAA AAAQPGSYPA
    61  LSAQAAREPA AFWGPLARDT LVWDTPYHTV WDCDFSTGKI GWFLGGQLNV SVNCLDQHVR
   121  KSPESVALIW ERDEPGTEVR ITYRELLETT CRLANTLKRH GVHRGDRVAI YMPVSPLAVA
   181  AMLACARIGA VHTVIFAGFS AESLAGRIND AKCKVVITFN QGLRGGRVVE LKKIVDEAVK
   241  HCPTVQHVLV AHRTDNKVHM GDLDVPLEQE MAKEDPVCAP ESMGSEDMLF MLYTSGSTGM
   301  PKGIVHTQAG YLLYAALTHK LVFDHQPGDI FGCVADIGWI TGHSYVVYGP LCNGATSVLF
   361  ESTPVYPNAG RYWETVERLK INQFYGAPTA VRLLLKYGDA WVKKYDRSSL RTLGSVGEPI
   421  NCEAWEWLHR VVGDSRCTLV DTWWQTETGG ICIAPRPSEE GAEILPAMAM RPFFGIVPVL
   481  MDEKGSVVEG SNVSGALCIS QAWPGMARTI YGDHQRFVDA YFKAYPGYYF TGDGAYRTEG
   541  GYYQITGRMD DVINISGHRL GTAEIEDAIA DHPAVPESAV IGYPHDIKGE AAFAFIVVKD
   601  SAGDSDVVVQ ELKSMVATKI AKYAVPDEIL VVKRLPKTRS GKVMRRLLRK IITSEAQELG
   661  DTTTLEDPSI IAEILSVYQK CKDKQAAAK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ACSS1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.25

Highest tissue expression

170 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.76

gnomAD pLI

0

gnomAD missense Z

1.83

DepMap mean gene effect

0.07

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• acetate biosynthetic process
• acetyl-CoA biosynthetic process
• acetyl-CoA biosynthetic process from acetate
• ethanol catabolic process
• propionate biosynthetic process

Molecular functions

• acetate-CoA ligase activity
• AMP binding
• ATP binding
• propionate-CoA ligase activity

Cellular components

• mitochondrial matrix
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• AMP-dependent synthetase/ligase domain
• Acetate-CoA ligase
• AMP-binding, conserved site
• AMP-binding enzyme, C-terminal domain
• Acetyl-coenzyme A synthetase, N-terminal domain
• ANL, N-terminal domain
• AMP-binding enzyme domain superfamily
• AMP-binding enzyme
• AMP-binding enzyme C-terminal domain
• Acetyl-coenzyme A synthetase N-terminus

KeywordsUniProt

• Acetylation
• ATP-binding
• Ligase
• Lipid metabolism
• Mitochondrion
• Nucleotide-binding
• Transit peptide

InteractionsUniProt · HPA

Protein binding partners of ACSS1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• SIRT3