Seroatlas · Human Serome Atlas

ZNF397

Zinc finger protein 397

Also known as: MGC13250, ZN397_HUMAN, ZNF47, ZSCAN15

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8NF99
Gene: ZNF397
Ensembl: ENSG00000186812
Chromosome: 18
Canonical length: 534 aa
Protein class: Predicted intracellular proteins, Transcription factors
Subcellular location: Nucleoli,Plasma membrane,Microtubules,Cytosol

OverviewNCBI Gene

This gene encodes a protein with a N-terminal SCAN domain, and the longer isoform contains nine C2H2-type zinc finger repeats in the C-terminal domain. The protein localizes to centromeres during interphase and early prophase, and different isoforms can repress or activate transcription in transfection studies. Multiple transcript variants encoding different isoforms have been found for this gene. Additional variants have been described, but their biological validity has not been determined. [provided by RefSeq, Oct 2009]

Canonical amino-acid sequenceUniProt

534 residues, UniProt reviewed canonical sequence.

>Q8NF99|ZNF397
     1  MAVESGVIST LIPQDPPEQE LILVKVEDNF SWDEKFKQNG STQSCQELFR QQFRKFCYQE
    61  TPGPREALSR LQELCYQWLM PELHTKEQIL ELLVLEQFLS ILPEELQIWV QQHNPESGEE
   121  AVTLLEDLER EFDDPGQQVP ASPQGPAVPW KDLTCLRASQ ESTDIHLQPL KTQLKSWKPC
   181  LSPKSDCENS ETATKEGISE EKSQGLPQEP SFRGISEHES NLVWKQGSAT GEKLRSPSQG
   241  GSFSQVIFTN KSLGKRDLYD EAERCLILTT DSIMCQKVPP EERPYRCDVC GHSFKQHSSL
   301  TQHQRIHTGE KPYKCNQCGK AFSLRSYLII HQRIHSGEKA YECSECGKAF NQSSALIRHR
   361  KIHTGEKACK CNECGKAFSQ SSYLIIHQRI HTGEKPYECN ECGKTFSQSS KLIRHQRIHT
   421  GERPYECNEC GKAFRQSSEL ITHQRIHSGE KPYECSECGK AFSLSSNLIR HQRIHSGEEP
   481  YQCNECGKTF KRSSALVQHQ RIHSGDEAYI CNECGKAFRH RSVLMRHQRV HTIK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZNF397 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.48
Highest tissue expression: 23 nTPM

Expression across tissuesHPA

Tissue

retina: 23 nTPM
lymph node: 15 nTPM
spleen: 15 nTPM
breast: 15 nTPM
ovary: 15 nTPM
tonsil: 15 nTPM

Single-cell type

cardiomyocytes: 350 nCPM
oligodendrocytes: 123 nCPM
myonuclei: 118 nCPM
epicardial cells: 100 nCPM
late spermatids: 80 nCPM
adipocytes: 79 nCPM

Immune cell

naive B-cell: 11 nTPM
neutrophil: 11 nTPM
memory B-cell: 11 nTPM
basophil: 10 nTPM
non-classical monocyte: 9.4 nTPM
eosinophil: 8.3 nTPM

Brain region

white matter: 80 nTPM
basal ganglia: 59 nTPM
cerebral cortex: 56 nTPM
midbrain: 55 nTPM
medulla oblongata: 55 nTPM
hypothalamus: 54 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.8
gnomAD pLI: 0
gnomAD missense Z: 1.03
DepMap mean gene effect: -0.05
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ZNF397 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ZNF397 as an antibody target. Whether an autoantibody or antibody against ZNF397 could matter depends on whether native ZNF397 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ZNF397 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ZNF397 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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