Seroatlas · Human Serome Atlas

WDR74

WD repeat-containing protein 74

Also known as: FLJ10439, Nsa1, WDR74_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q6RFH5
Gene: WDR74
Ensembl: ENSG00000133316
Chromosome: 11
Canonical length: 385 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nucleoli

OverviewNCBI Gene

Involved in rRNA processing and ribosomal large subunit biogenesis. Located in nuclear exosome (RNase complex); nucleolus; and nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

385 residues, UniProt reviewed canonical sequence.

>Q6RFH5|WDR74
     1  MAAAAARWNH VWVGTETGIL KGVNLQRKQA ANFTAGGQPR REEAVSALCW GTGGETQMLV
    61  GCADRTVKHF STEDGIFQGQ RHCPGGEGMF RGLAQADGTL ITCVDSGILR VWHDKDKDTS
   121  SDPLLELRVG PGVCRMRQDP AHPHVVATGG KENALKIWDL QGSEEPVFRA KNVRNDWLDL
   181  RVPIWDQDIQ FLPGSQKLVT CTGYHQVRVY DPASPQRRPV LETTYGEYPL TAMTLTPGGN
   241  SVIVGNTHGQ LAEIDLRQGR LLGCLKGLAG SVRGLQCHPS KPLLASCGLD RVLRIHRIQN
   301  PRGLEHKVYL KSQLNCLLLS GRDNWEDEPQ EPQEPNKVPL EDTETDELWA SLEAAAKRKL
   361  SGLEQPQGAL QTRRRKKKRP GSTSP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against WDR74 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.32
Highest tissue expression: 119 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 119 nTPM
skeletal muscle: 61 nTPM
thymus: 58 nTPM
pancreas: 32 nTPM
retina: 31 nTPM
skin: 31 nTPM

Single-cell type

plasma cells: 199 nCPM
gastric chief cells: 162 nCPM
mucous neck cells: 147 nCPM
pdcs: 136 nCPM
innate lymphoid cells: 132 nCPM
esophageal basal cells: 104 nCPM

Immune cell

plasmacytoid DC: 154 nTPM
classical monocyte: 141 nTPM
naive B-cell: 121 nTPM
myeloid DC: 105 nTPM
NK-cell: 93 nTPM
memory B-cell: 66 nTPM

Brain region

choroid plexus: 1,916 nTPM
white matter: 1,590 nTPM
cerebellum: 1,407 nTPM
basal ganglia: 1,133 nTPM
cerebral cortex: 1,084 nTPM
spinal cord: 1,057 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.12
gnomAD pLI: 0
gnomAD missense Z: 0.92
DepMap mean gene effect: -1.8
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of WDR74 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads WDR74 as an antibody target. Whether an autoantibody or antibody against WDR74 could matter depends on whether native WDR74 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

WDR74 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label WDR74 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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