UNC13B

Protein identityUniProt · HPA

UniProt accession

O14795

Gene

UNC13B

Ensembl

ENSG00000198722

Chromosome

9

Canonical length

1591 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm,Golgi apparatus,Vesicles,Intermediate filaments

OverviewNCBI Gene

This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1591 residues, UniProt reviewed canonical sequence.

>O14795|UNC13B
     1  MSLLCVRVKR AKFQGSPDKF NTYVTLKVQN VKSTTVAVRG DQPSWEQDFM FEISRLDLGL
    61  SVEVWNKGLI WDTMVGTVWI ALKTIRQSDE EGPGEWSTLE AETLMKDDEI CGTRNPTPHK
   121  ILLDTRFELP FDIPEEEARY WTYKWEQINA LGADNEYSSQ EESQRKPLPT AAAQCSFEDP
   181  DSAVDDRDSD YRSETSNSFP PPYHTASQPN ASVHQFPVPV RSPQQLLLQG SSRDSCNDSM
   241  QSYDLDYPER RAISPTSSSR YGSSCNVSQG SSQLSELDQY HEQDDDHRET DSIHSCHSSH
   301  SLSRDGQAGF GEQEKPLEVT GQAEKEAACE PKEMKEDATT HPPPDLVLQK DHFLGPQESF
   361  PEENASSPFT QARAHWIRAV TKVRLQLQEI PDDGDPSLPQ WLPEGPAGGL YGIDSMPDLR
   421  RKKPLPLVSD LSLVQSRKAG ITSAMATRTS LKDEELKSHV YKKTLQALIY PISCTTPHNF
   481  EVWTATTPTY CYECEGLLWG IARQGMRCSE CGVKCHEKCQ DLLNADCLQR AAEKSCKHGA
   541  EDRTQNIIMA MKDRMKIRER NKPEIFEVIR DVFTVNKAAH VQQMKTVKQS VLDGTSKWSA
   601  KITITVVCAQ GLQAKDKTGS SDPYVTVQVS KTKKRTKTIF GNLNPVWEEK FHFECHNSSD
   661  RIKVRVWDED DDIKSRVKQR LKRESDDFLG QTIIEVRTLS GEMDVWYNLE KRTDKSAVSG
   721  AIRLQISVEI KGEEKVAPYH VQYTCLHENL FHYLTDIQGS GGVRIPEARG DDAWKVYFDE
   781  TAQEIVDEFA MRYGIESIYQ AMTHFACLSS KYMCPGVPAV MSTLLANINA YYAHTTASTN
   841  VSASDRFAAS NFGKERFVKL LDQLHNSLRI DLSTYRNNFP AGSPERLQDL KSTVDLLTSI
   901  TFFRMKVQEL QSPPRASQVV KDCVKACLNS TYEYIFNNCH DLYSRQYQLK QELPPEEQGP
   961  SIRNLDFWPK LITLIVSIIE EDKNSYTPVL NQFPQELNVG KVSAEVMWHL FAQDMKYALE
  1021  EHEKDHLCKS ADYMNLHFKV KWLHNEYVRD LPVLQGQVPE YPAWFEQFVL QWLDENEDVS
  1081  LEFLRGALER DKKDGFQQTS EHALFSCSVV DVFTQLNQSF EIIRKLECPD PSILAHYMRR
  1141  FAKTIGKVLM QYADILSKDF PAYCTKEKLP CILMNNVQQL RVQLEKMFEA MGGKELDLEA
  1201  ADSLKELQVK LNTVLDELSM VFGNSFQVRI DECVRQMADI LGQVRGTGNA SPDARASAAQ
  1261  DADSVLRPLM DFLDGNLTLF ATVCEKTVLK RVLKELWRVV MNTMERMIVL PPLTDQTGTQ
  1321  LIFTAAKELS HLSKLKDHMV REETRNLTPK QCAVLDLALD TIKQYFHAGG NGLKKTFLEK
  1381  SPDLQSLRYA LSLYTQTTDT LIKTFVRSQT TQGSGVDDPV GEVSIQVDLF THPGTGEHKV
  1441  TVKVVAANDL KWQTAGMFRP FVEVTMVGPH QSDKKRKFTT KSKSNNWAPK YNETFHFLLG
  1501  NEEGPESYEL QICVKDYCFA REDRVLGLAV MPLRDVTAKG SCACWCPLGR KIHMDETGLT
  1561  ILRILSQRSN DEVAREFVKL KSESRSTEEG S

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against UNC13B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.34

Highest tissue expression

38 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.59

gnomAD pLI

0

gnomAD missense Z

1.45

DepMap mean gene effect

-0.1

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• acrosomal vesicle exocytosis
• cellular response to glucose stimulus
• chemical synaptic transmission
• dense core granule priming
• neuronal dense core vesicle exocytosis
• positive regulation of apoptotic process
• positive regulation of inhibitory postsynaptic potential
• positive regulation of protein secretion
• synaptic transmission, glutamatergic
• synaptic vesicle docking
• synaptic vesicle priming
• positive regulation of synaptic vesicle priming

Molecular functions

• calcium ion binding
• calmodulin binding
• diacylglycerol binding
• GTP-dependent protein binding
• phospholipid binding
• small GTPase binding
• syntaxin-1 binding
• zinc ion binding

Cellular components

• cytosol
• Golgi apparatus
• membrane
• neuromuscular junction
• plasma membrane
• presynaptic active zone
• presynaptic membrane
• synaptic vesicle membrane
• terminal bouton

Protein domainsUniProt · Pfam · InterPro

• C2 domain
• Protein kinase C-like, phorbol ester/diacylglycerol-binding domain
• MUN domain
• Munc13 homology 1
• Mammalian uncoordinated homology 13, domain 2
• Protein Unc-13
• C2 domain superfamily
• Protein Unc-13, C2B domain
• C1-like domain superfamily
• Phorbol esters/diacylglycerol binding domain (C1 domain)
• C2 domain
• MUN domain

KeywordsUniProt

• Calcium
• Cell membrane
• Coiled coil
• Cytoplasm
• Exocytosis
• Membrane
• Metal-binding
• Phosphoprotein
• Repeat
• Synapse
• Zinc
• Zinc-finger

InteractionsUniProt · HPA

Protein binding partners of UNC13B in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• RIMS1
• CALM1