UBE2J1

Protein identityUniProt · HPA

UniProt accession

Q9Y385

Gene

UBE2J1

Ensembl

ENSG00000198833

Chromosome

6

Canonical length

318 aa

Protein class

Enzymes, Metabolic proteins, Predicted membrane proteins

OverviewNCBI Gene

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

318 residues, UniProt reviewed canonical sequence.

>Q9Y385|UBE2J1
     1  METRYNLKSP AVKRLMKEAA ELKDPTDHYH AQPLEDNLFE WHFTVRGPPD SDFDGGVYHG
    61  RIVLPPEYPM KPPSIILLTA NGRFEVGKKI CLSISGHHPE TWQPSWSIRT ALLAIIGFMP
   121  TKGEGAIGSL DYTPEERRAL AKKSQDFCCE GCGSAMKDVL LPLKSGSDSS QADQEAKELA
   181  RQISFKAEVN SSGKTISESD LNHSFSLTDL QDDIPTTFQG ATASTSYGLQ NSSAASFHQP
   241  TQPVAKNTSM SPRQRRAQQQ SQRRLSTSPD VIQGHQPRDN HTDHGGSAVL IVILTLALAA
   301  LIFRRIYLAN EYIFDFEL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against UBE2J1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.41

Highest tissue expression

113 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.5

gnomAD pLI

0.44

gnomAD missense Z

1.03

DepMap mean gene effect

-0.04

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• ERAD pathway
• negative regulation of retrograde protein transport, ER to cytosol
• protein N-linked glycosylation via asparagine
• protein polyubiquitination
• regulation of macrophage cytokine production
• regulation of tumor necrosis factor production
• spermatid development

Molecular functions

• ATP binding
• ubiquitin conjugating enzyme activity
• ubiquitin protein ligase binding

Cellular components

• endoplasmic reticulum membrane
• Hrd1p ubiquitin ligase complex
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Ubiquitin-conjugating (UBC), catalytic core domain
• Ubiquitin-conjugating enzyme/RWD-like
• Ubiquitin-conjugating enzyme E2-like
• Ubiquitin-conjugating enzyme

KeywordsUniProt

• ATP-binding
• Endoplasmic reticulum
• Membrane
• Nucleotide-binding
• Phosphoprotein
• Transferase
• Transmembrane
• Transmembrane helix
• Ubl conjugation
• Ubl conjugation pathway

InteractionsUniProt · HPA

Protein binding partners of UBE2J1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• RNF26
• DERL1
• STIM1
• SYVN1