Seroatlas · Human Serome Atlas

TMX3

Protein disulfide-isomerase TMX3

Also known as: FLJ20793, KIAA1830, PDIA13, TMX3_HUMAN, TXNDC10

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96JJ7
Gene: TMX3
Ensembl: ENSG00000166479
Chromosome: 18
Canonical length: 454 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins, Transporters
Secretome location: Intracellular and membrane

OverviewNCBI Gene

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The canonical protein encoded by this gene has an N-terminal ER-signal sequence, a catalytically active thioredoxin domain, one transmembrane domain and a C-terminal ER-retention sequence. This gene is expressed in many tissues but has its highest expression in heart and skeletal muscle. It is expressed in the retinal neuroepithelium and lens epithelium in the developing murine eye and haploinsufficiency of this gene in humans and zebrafish is associated with microphthalmia. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2017]

Canonical amino-acid sequenceUniProt

454 residues, UniProt reviewed canonical sequence.

>Q96JJ7|TMX3
     1  MAAWKSWTAL RLCATVVVLD MVVCKGFVED LDESFKENRN DDIWLVDFYA PWCGHCKKLE
    61  PIWNEVGLEM KSIGSPVKVG KMDATSYSSI ASEFGVRGYP TIKLLKGDLA YNYRGPRTKD
   121  DIIEFAHRVS GALIRPLPSQ QMFEHMQKRH RVFFVYVGGE SPLKEKYIDA ASELIVYTYF
   181  FSASEEVVPE YVTLKEMPAV LVFKDETYFV YDEYEDGDLS SWINRERFQN YLAMDGFLLY
   241  ELGDTGKLVA LAVIDEKNTS VEHTRLKSII QEVARDYRDL FHRDFQFGHM DGNDYINTLL
   301  MDELTVPTVV VLNTSNQQYF LLDRQIKNVE DMVQFINNIL DGTVEAQGGD SILQRLKRIV
   361  FDAKSTIVSI FKSSPLMGCF LFGLPLGVIS IMCYGIYTAD TDGGYIEERY EVSKSENENQ
   421  EQIEESKEQQ EPSSGGSVVP TVQEPKDVLE KKKD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TMX3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.35
Highest tissue expression: 26 nTPM

Expression across tissuesHPA

Tissue

lymph node: 26 nTPM
retina: 24 nTPM
appendix: 23 nTPM
thymus: 22 nTPM
adipose tissue: 21 nTPM
spleen: 21 nTPM

Single-cell type

alveolar cells type 1: 85 nCPM
brain excitatory neurons: 77 nCPM
pituicytes/fscs: 75 nCPM
microglia: 74 nCPM
pdcs: 74 nCPM
fibro-adipogenic progenitors: 70 nCPM

Immune cell

plasmacytoid DC: 6.4 nTPM
MAIT T-cell: 5.5 nTPM
NK-cell: 4.9 nTPM
intermediate monocyte: 4.7 nTPM
myeloid DC: 4.7 nTPM
T-reg: 4.5 nTPM

Brain region

cerebellum: 25 nTPM
cerebral cortex: 24 nTPM
hypothalamus: 24 nTPM
basal ganglia: 22 nTPM
hippocampal formation: 22 nTPM
pons: 22 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.59
gnomAD pLI: 0
gnomAD missense Z: 1.19
DepMap mean gene effect: 0.14
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TMX3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TMX3 as an antibody target. Whether an autoantibody or antibody against TMX3 could matter depends on whether native TMX3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TMX3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TMX3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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