Seroatlas · Human Serome Atlas

TMED4

Transmembrane emp24 domain-containing protein 4

Also known as: HNLF, p24a3, p24alpha3, TMED4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q7Z7H5
Gene
TMED4
Ensembl
ENSG00000158604
Chromosome
7
Canonical length
227 aa
Protein class
Predicted intracellular proteins, Predicted membrane proteins
Secretome location
Intracellular and membrane

OverviewNCBI Gene

Involved in positive regulation of canonical NF-kappaB signal transduction. Predicted to be located in endoplasmic reticulum membrane. Predicted to be active in several cellular components, including COPII-coated ER to Golgi transport vesicle; Golgi apparatus; and endoplasmic reticulum-Golgi intermediate compartment. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

227 residues, UniProt reviewed canonical sequence.

>Q7Z7H5|TMED4
     1  MAGVGAGPLR AMGRQALLLL ALCATGAQGL YFHIGETEKR CFIEEIPDET MVIGNYRTQM
    61  WDKQKEVFLP STPGLGMHVE VKDPDGKVVL SRQYGSEGRF TFTSHTPGDH QICLHSNSTR
   121  MALFAGGKLR VHLDIQVGEH ANNYPEIAAK DKLTELQLRA RQLLDQVEQI QKEQDYQRYR
   181  EERFRLTSES TNQRVLWWSI AQTVILILTG IWQMRHLKSF FEAKKLV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TMED4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.39
Highest tissue expression
131 nTPM

Expression across tissuesHPA

Tissue

  • parathyroid gland: 131 nTPM
  • thyroid gland: 107 nTPM
  • small intestine: 87 nTPM
  • retina: 85 nTPM
  • duodenum: 83 nTPM
  • kidney: 82 nTPM

Single-cell type

  • enterocytes: 241 nCPM
  • cytotrophoblasts: 206 nCPM
  • fallopian tube ciliated cells: 195 nCPM
  • syncytiotrophoblasts: 187 nCPM
  • plasma cells: 176 nCPM
  • lacrimal acinar cells: 161 nCPM

Immune cell

  • plasmacytoid DC: 120 nTPM
  • MAIT T-cell: 103 nTPM
  • basophil: 95 nTPM
  • eosinophil: 87 nTPM
  • gdT-cell: 85 nTPM
  • memory CD4 T-cell: 85 nTPM

Brain region

  • white matter: 86 nTPM
  • choroid plexus: 84 nTPM
  • hypothalamus: 73 nTPM
  • medulla oblongata: 72 nTPM
  • basal ganglia: 71 nTPM
  • midbrain: 66 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about TMED4.

Disease | ImmuneIEDB

Conditions an epitope on TMED4 was assayed in.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.66
gnomAD pLI
0.24
gnomAD missense Z
0.74
DepMap mean gene effect
0
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 11% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TMED4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TMED4 as an antibody target. Whether an autoantibody or antibody against TMED4 could matter depends on whether native TMED4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TMED4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TMED4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TMED4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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