Seroatlas · Human Serome Atlas

TGS1

Trimethylguanosine synthase

Also known as: NCOA6IP, PIMT, TGS1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96RS0
Gene: TGS1
Ensembl: ENSG00000137574
Chromosome: 8
Canonical length: 853 aa
Protein class: Metabolic proteins, Predicted intracellular proteins
Subcellular location: Cytosol
Quaternary structure: Homooligomer

OverviewNCBI Gene

Enables RNA cap trimethylguanosine synthase activity. Involved in 7-methylguanosine cap hypermethylation. Located in cytosol and nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

853 residues, UniProt reviewed canonical sequence.

>Q96RS0|TGS1
     1  MCCEKWSRVA EMFLFIEERE DCKILCLCSR AFVEDRKLYN LGLKGYYIRD SGNNSGDQAT
    61  EEEEGGYSCG TAESHDSKGI GLDESELDSE AELMRSMGLP LQFGRITAHK DFEVSMNTRN
   121  KVKIKKKKHQ KKYLDEIVQE SWRKEYEEDD ILASDDPSSI EQYENTRTYE LQSKKDTETE
   181  NPPVENTLSP KLEITEKWEK YWNEYGGGLL WQSWQEKHPG QALSSEPWNF PDTKEEWEQH
   241  YSQLYWYYLE QFQYWEAQGW TFDASQSCDT DTYTSKTEAD DKNDEKCMKV DLVSFPSSPI
   301  MVDNDSSGTS DKDHSEILDG ISNIKLNSEE VTQSQLDSCT SHDGHQQLSE VSSKRECPAS
   361  GQSEPRNGGT NEESNSSGNT NTDPPAEDSQ KSSGANTSKD RPHASGTDGD ESEEDPPEHK
   421  PSKLKRSHEL DIDENPASDF DDSGSLLGFK YGSGQKYGGI PNFSHRQVRY LEKNVKLKSK
   481  YLDMRRQIKM KNKHIFFTKE SEKPFFKKSK ILSKVEKFLT WVNKPMDEEA SQESSSHDNV
   541  HDASTSSDSE EQDMSVKKGD DLLETNNPEP EKCQSVSSAG ELETENYERD SLLATVPDEQ
   601  DCVTQEVPDS RQAETEAEVK KKKNKKKNKK VNGLPPEIAA VPELAKYWAQ RYRLFSRFDD
   661  GIKLDREGWF SVTPEKIAEH IAGRVSQSFK CDVVVDAFCG VGGNTIQFAL TGMRVIAIDI
   721  DPVKIALARN NAEVYGIADK IEFICGDFLL LASFLKADVV FLSPPWGGPD YATAETFDIR
   781  TMMSPDGFEI FRLSKKITNN IVYFLPRNAD IDQVASLAGP GGQVEIEQNF LNNKLKTITA
   841  YFGDLIRRPA SET

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TGS1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.49
Highest tissue expression: 8.8 nTPM

Expression across tissuesHPA

Tissue

thymus: 8.8 nTPM
lymph node: 8 nTPM
tonsil: 8 nTPM
bone marrow: 6.8 nTPM
urinary bladder: 6.8 nTPM
parathyroid gland: 6.4 nTPM

Single-cell type

early primary spermatocytes: 144 nCPM
erythrocyte progenitors: 124 nCPM
renal collecting duct principal cells: 123 nCPM
epididymal principal cells: 87 nCPM
differentiating spermatogonia: 83 nCPM
mast cells: 78 nCPM

Immune cell

MAIT T-cell: 6 nTPM
naive CD4 T-cell: 5.2 nTPM
naive CD8 T-cell: 5.2 nTPM
plasmacytoid DC: 5.2 nTPM
gdT-cell: 5.1 nTPM
basophil: 5 nTPM

Brain region

cerebellum: 11 nTPM
choroid plexus: 8.1 nTPM
white matter: 6.9 nTPM
basal ganglia: 6.6 nTPM
cerebral cortex: 6.6 nTPM
hypothalamus: 6.2 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about TGS1.

Disease | ImmuneIEDB

Conditions an epitope on TGS1 was assayed in.

skin melanoma T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.91
gnomAD pLI: 0
gnomAD missense Z: -1.16
DepMap mean gene effect: -0.76
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 13% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

RNA methyltransferase activity
RNA cap trimethylguanosine synthase activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

S-adenosyl-L-methionine-dependent methyltransferase superfamily
RNA cap guanine-N2 methyltransferase
RNA cap guanine-N2 methyltransferase

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TGS1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TGS1 as an antibody target. Whether an autoantibody or antibody against TGS1 could matter depends on whether native TGS1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TGS1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TGS1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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