Seroatlas · Human Serome Atlas

SPSB3

SPRY domain-containing SOCS box protein 3

Also known as: C16orf31, SPSB3_HUMAN, SSB-3

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q6PJ21
Gene
SPSB3
Ensembl
ENSG00000162032
Chromosome
16
Canonical length
355 aa
Protein class
Predicted intracellular proteins
Subcellular location
Mitochondria

OverviewNCBI Gene

Enables ubiquitin-like ligase-substrate adaptor activity. Involved in several processes, including negative regulation of cGAS/STING signaling pathway; negative regulation of epithelial to mesenchymal transition; and protein K48-linked ubiquitination. Part of Cul5-RING ubiquitin ligase complex. Is active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

355 residues, UniProt reviewed canonical sequence.

>Q6PJ21|SPSB3
     1  MARRPRNSRA WHFVLSAARR DADARAVALA GSTNWGYDSD GQHSDSDSDP EYSTLPPSIP
    61  SAVPVTGESF CDCAGQSEAS FCSSLHSAHR GRDCRCGEED EYFDWVWDDL NKSSATLLSC
   121  DNRKVSFHME YSCGTAAIRG TKELGEGQHF WEIKMTSPVY GTDMMVGIGT SDVDLDKYRH
   181  TFCSLLGRDE DSWGLSYTGL LHHKGDKTSF SSRFGQGSII GVHLDTWHGT LTFFKNRKCI
   241  GVAATKLQNK RFYPMVCSTA ARSSMKVTRS CASATSLQYL CCHRLRQLRP DSGDTLEGLP
   301  LPPGLKQVLH NKLGWVLSMS CSRRKAPVSD PQAATSAHPS SREPRPCQRK RCRRT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPSB3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.4
Highest tissue expression
99 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 99 nTPM
  • cerebellum: 80 nTPM
  • skin: 56 nTPM
  • adrenal gland: 51 nTPM
  • pancreas: 50 nTPM
  • liver: 50 nTPM

Single-cell type

  • esophageal apical cells: 619 nCPM
  • oocytes: 166 nCPM
  • breast lactating cells: 142 nCPM
  • late spermatids: 121 nCPM
  • cardiomyocytes: 118 nCPM
  • ovarian stromal cells: 108 nCPM

Immune cell

  • basophil: 113 nTPM
  • T-reg: 101 nTPM
  • neutrophil: 92 nTPM
  • eosinophil: 84 nTPM
  • MAIT T-cell: 81 nTPM
  • gdT-cell: 68 nTPM

Brain region

  • cerebellum: 43 nTPM
  • white matter: 32 nTPM
  • medulla oblongata: 28 nTPM
  • cerebral cortex: 28 nTPM
  • thalamus: 27 nTPM
  • midbrain: 26 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.71
gnomAD pLI
0.04
gnomAD missense Z
0.78
DepMap mean gene effect
0.03
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SPSB3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SPSB3 as an antibody target. Whether an autoantibody or antibody against SPSB3 could matter depends on whether native SPSB3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SPSB3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SPSB3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/SPSB3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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