Seroatlas · Human Serome Atlas

SPRED3

Sprouty-related, EVH1 domain-containing protein 3

Also known as: SPRE3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q2MJR0
Gene: SPRED3
Ensembl: ENSG00000188766
Chromosome: 19
Canonical length: 410 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins
Subcellular location: Nucleoplasm,Plasma membrane

OverviewNCBI Gene

This gene encodes a protein with a C-terminal Sprouty-like cysteine-rich domain (SRY) and an N-terminal Ena/Vasodilator-stimulated phosphoprotein (VASP) homology-1 (EVH-1) domain. The encoded protein is a member of a family of proteins that negatively regulates mitogen-activated protein (MAP) kinase signaling, particularly during organogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]

Canonical amino-acid sequenceUniProt

410 residues, UniProt reviewed canonical sequence.

>Q2MJR0|SPRED3
     1  MVRVRAVVMA RDDSSGGWLP VGGGGLSQVS VCRVRGARPE GGARQGHYVI HGERLRDQKT
    61  TLECTLKPGL VYNKVNPIFH HWSLGDCKFG LTFQSPAEAD EFQKSLLAAL AALGRGSLTP
   121  SSSSSSSSPS QDTAETPCPL TSHVDSDSSS SHSRQETPPS AAAAPIITME SASGFGPTTP
   181  PQRRRSSAQS YPPLLPFTGI PEPSEPLAGA GGLGWGGRGY EDYRRSGPPA PLALSTCVVR
   241  FAKTGALRGA ALGPPAALPA PLTEAAPPAP PARPPPGPGP SSAPAKASPE AEEAARCVHC
   301  RALFRRRADG RGGRCAEAPD PGRLLVRRLS CLWCAESLLY HCLSDAEGDF SDPCACEPGH
   361  PRPAARWAAL AALSLAVPCL CCYAPLRACH WVAARCGCAG CGGRHEEAAR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPRED3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.5
Highest tissue expression: 3.5 nTPM

Expression across tissuesHPA

Tissue

cerebral cortex: 3.5 nTPM
hippocampal formation: 3.1 nTPM
amygdala: 2.4 nTPM
pituitary gland: 2.4 nTPM
hypothalamus: 2.3 nTPM
blood vessel: 2 nTPM

Single-cell type

pancreatic islet cells: 31 nCPM
neuroendocrine cells: 27 nCPM
other brain neurons: 16 nCPM
pituicytes/fscs: 13 nCPM
oligodendrocyte progenitor cells: 12 nCPM
lactotrophs: 12 nCPM

Immune cell

basophil: 0.4 nTPM
neutrophil: 0.3 nTPM
classical monocyte: 0.1 nTPM
gdT-cell: 0.1 nTPM
memory CD4 T-cell: 0.1 nTPM
naive B-cell: 0.1 nTPM

Brain region

hypothalamus: 21 nTPM
midbrain: 16 nTPM
cerebral cortex: 16 nTPM
pons: 15 nTPM
hippocampal formation: 15 nTPM
medulla oblongata: 14 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.69
gnomAD pLI: 0.21
gnomAD missense Z: 1.43
DepMap mean gene effect: -0.1
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

protein kinase binding

Cellular components

plasma membrane

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SPRED3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

ZDHHC17

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SPRED3 as an antibody target. Whether an autoantibody or antibody against SPRED3 could matter depends on whether native SPRED3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SPRED3 is annotated at the cell surface, where native SPRED3 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label SPRED3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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