SPOUT1

Protein identityUniProt · HPA

UniProt accession

Q5T280

Gene

SPOUT1

Ensembl

ENSG00000198917

Chromosome

9

Canonical length

376 aa

Protein class

Predicted intracellular proteins

Quaternary structure

Homodimer

OverviewNCBI Gene

Enables miRNA binding activity. Involved in maintenance of centrosome location and miRNA processing. Acts upstream of with a positive effect on post-transcriptional regulation of gene expression. Located in kinetochore; mitotic spindle; and spindle pole centrosome. [provided by Alliance of Genome Resources, Apr 2025]

Canonical amino-acid sequenceUniProt

376 residues, UniProt reviewed canonical sequence.

>Q5T280|SPOUT1
     1  MAERGRKRPC GPGEHGQRIE WRKWKQQKKE EKKKWKDLKL MKKLERQRAQ EEQAKRLEEE
    61  EAAAEKEDRG RPYTLSVALP GSILDNAQSP ELRTYLAGQI ARACAIFCVD EIVVFDEEGQ
   121  DAKTVEGEFT GVGKKGQACV QLARILQYLE CPQYLRKAFF PKHQDLQFAG LLNPLDSPHH
   181  MRQDEESEFR EGIVVDRPTR PGHGSFVNCG MKKEVKIDKN LEPGLRVTVR LNQQQHPDCK
   241  TYHGKVVSSQ DPRTKAGLYW GYTVRLASCL SAVFAEAPFQ DGYDLTIGTS ERGSDVASAQ
   301  LPNFRHALVV FGGLQGLEAG ADADPNLEVA EPSVLFDLYV NTCPGQGSRT IRTEEAILIS
   361  LAALQPGLIQ AGARHT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPOUT1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.31

Highest tissue expression

43 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about SPOUT1.

Disease | AllUniProt

Conditions SPOUT1 is implicated in, by any mechanism.

• Neurodevelopmental disorder with poor growth, seizures, and brain abnormalities (NEDGSB) MIM:621154

Disease | GeneticClinVar

6 pathogenic / likely-pathogenic of 56 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Neurodevelopmental disorder
• Neurodevelopmental disorder with poor growth, seizures, and brain abnormalities
• Undetermined early-onset epileptic encephalopathy

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1

gnomAD pLI

0

DepMap mean gene effect

-1.36

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cell division
• maintenance of centrosome location
• miRNA processing
• post-transcriptional regulation of gene expression
• rRNA base methylation

Molecular functions

• methyltransferase activity
• miRNA binding
• RNA binding
• RNA methyltransferase activity
• rRNA (uridine-N3-)-methyltransferase activity
• S-adenosyl-L-methionine binding

Cellular components

• kinetochore
• mitotic spindle
• spindle pole centrosome

Protein domainsUniProt · Pfam · InterPro

• Nucleic acid-binding, OB-fold
• tRNA (guanine-N1-)-methyltransferase, N-terminal
• Alpha/beta knot methyltransferases
• Putative RNA methyltransferase C9orf114-like
• Putative RNA methyltransferase

KeywordsUniProt

• Cell cycle
• Cell division
• Centromere
• Chromosome
• Cytoplasm
• Cytoskeleton
• Epilepsy
• Intellectual disability
• Kinetochore
• Methyltransferase
• Mitosis
• RNA-binding
• rRNA processing
• S-adenosyl-L-methionine
• Transferase

InteractionsUniProt · HPA

Protein binding partners of SPOUT1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• INCA1