Seroatlas · Human Serome Atlas

SH3RF1

E3 ubiquitin-protein ligase SH3RF1

Also known as: KIAA1494, POSH, RNF142, SH3MD2, SH3R1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q7Z6J0
Gene
SH3RF1
Ensembl
ENSG00000154447
Chromosome
4
Canonical length
888 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location
Vesicles,Intermediate filaments

OverviewNCBI Gene

This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as an ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

888 residues, UniProt reviewed canonical sequence.

>Q7Z6J0|SH3RF1
     1  MDESALLDLL ECPVCLERLD ASAKVLPCQH TFCKRCLLGI VGSRNELRCP ECRTLVGSGV
    61  EELPSNILLV RLLDGIKQRP WKPGPGGGSG TNCTNALRSQ SSTVANCSSK DLQSSQGGQQ
   121  PRVQSWSPPV RGIPQLPCAK ALYNYEGKEP GDLKFSKGDI IILRRQVDEN WYHGEVNGIH
   181  GFFPTNFVQI IKPLPQPPPQ CKALYDFEVK DKEADKDCLP FAKDDVLTVI RRVDENWAEG
   241  MLADKIGIFP ISYVEFNSAA KQLIEWDKPP VPGVDAGECS SAAAQSSTAP KHSDTKKNTK
   301  KRHSFTSLTM ANKSSQASQN RHSMEISPPV LISSSNPTAA ARISELSGLS CSAPSQVHIS
   361  TTGLIVTPPP SSPVTTGPSF TFPSDVPYQA ALGTLNPPLP PPPLLAATVL ASTPPGATAA
   421  AAAAGMGPRP MAGSTDQIAH LRPQTRPSVY VAIYPYTPRK EDELELRKGE MFLVFERCQD
   481  GWFKGTSMHT SKIGVFPGNY VAPVTRAVTN ASQAKVPMST AGQTSRGVTM VSPSTAGGPA
   541  QKLQGNGVAG SPSVVPAAVV SAAHIQTSPQ AKVLLHMTGQ MTVNQARNAV RTVAAHNQER
   601  PTAAVTPIQV QNAAGLSPAS VGLSHHSLAS PQPAPLMPGS ATHTAAISIS RASAPLACAA
   661  AAPLTSPSIT SASLEAEPSG RIVTVLPGLP TSPDSASSAC GNSSATKPDK DSKKEKKGLL
   721  KLLSGASTKR KPRVSPPASP TLEVELGSAE LPLQGAVGPE LPPGGGHGRA GSCPVDGDGP
   781  VTTAVAGAAL AQDAFHRKAS SLDSAVPIAP PPRQACSSLG PVLNESRPVV CERHRVVVSY
   841  PPQSEAELEL KEGDIVFVHK KREDGWFKGT LQRNGKTGLF PGSFVENI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SH3RF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.58
Highest tissue expression
23 nTPM

Expression across tissuesHPA

Tissue

  • stomach: 23 nTPM
  • thyroid gland: 19 nTPM
  • salivary gland: 15 nTPM
  • colon: 14 nTPM
  • prostate: 14 nTPM
  • duodenum: 13 nTPM

Single-cell type

  • salivary acinar cells: 859 nCPM
  • podocytes: 847 nCPM
  • ocular epithelial cells: 685 nCPM
  • renal connecting tubule cells: 655 nCPM
  • urothelial cells: 623 nCPM
  • distal convoluted tubule cells: 607 nCPM

Immune cell

  • non-classical monocyte: 6.8 nTPM
  • intermediate monocyte: 4.2 nTPM
  • myeloid DC: 2.5 nTPM
  • classical monocyte: 1 nTPM
  • total PBMC: 0.6 nTPM
  • memory B-cell: 0.3 nTPM

Brain region

  • hippocampal formation: 37 nTPM
  • white matter: 26 nTPM
  • cerebral cortex: 26 nTPM
  • basal ganglia: 20 nTPM
  • midbrain: 20 nTPM
  • thalamus: 20 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.51
gnomAD pLI
0.01
gnomAD missense Z
1.51
DepMap mean gene effect
0.04
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SH3RF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SH3RF1 as an antibody target. Whether an autoantibody or antibody against SH3RF1 could matter depends on whether native SH3RF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SH3RF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SH3RF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/SH3RF1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...