Seroatlas · Human Serome Atlas

SEMA4D

Semaphorin-4D

Also known as: C9orf164, CD100, coll-4, FLJ39737, SEM4D_HUMAN, SEMAJ

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q92854
Gene
SEMA4D
Ensembl
ENSG00000187764
Chromosome
9
Canonical length
862 aa
Protein class
CD markers, Plasma proteins, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location
Nucleoplasm,Vesicles,Plasma membrane,Primary cilium transition zone,Centrosome,Basal body
Quaternary structure
Homodimer

OverviewNCBI Gene

Enables identical protein binding activity; semaphorin receptor binding activity; and transmembrane signaling receptor activity. Involved in several processes, including positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction; regulation of neuron projection development; and regulation of primary metabolic process. Located in microtubule organizing center; nucleoplasm; and plasma membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

862 residues, UniProt reviewed canonical sequence.

>Q92854|SEMA4D
     1  MRMCTPIRGL LMALAVMFGT AMAFAPIPRI TWEHREVHLV QFHEPDIYNY SALLLSEDKD
    61  TLYIGAREAV FAVNALNISE KQHEVYWKVS EDKKAKCAEK GKSKQTECLN YIRVLQPLSA
   121  TSLYVCGTNA FQPACDHLNL TSFKFLGKNE DGKGRCPFDP AHSYTSVMVD GELYSGTSYN
   181  FLGSEPIISR NSSHSPLRTE YAIPWLNEPS FVFADVIRKS PDSPDGEDDR VYFFFTEVSV
   241  EYEFVFRVLI PRIARVCKGD QGGLRTLQKK WTSFLKARLI CSRPDSGLVF NVLRDVFVLR
   301  SPGLKVPVFY ALFTPQLNNV GLSAVCAYNL STAEEVFSHG KYMQSTTVEQ SHTKWVRYNG
   361  PVPKPRPGAC IDSEARAANY TSSLNLPDKT LQFVKDHPLM DDSVTPIDNR PRLIKKDVNY
   421  TQIVVDRTQA LDGTVYDVMF VSTDRGALHK AISLEHAVHI IEETQLFQDF EPVQTLLLSS
   481  KKGNRFVYAG SNSGVVQAPL AFCGKHGTCE DCVLARDPYC AWSPPTATCV ALHQTESPSR
   541  GLIQEMSGDA SVCPDKSKGS YRQHFFKHGG TAELKCSQKS NLARVFWKFQ NGVLKAESPK
   601  YGLMGRKNLL IFNLSEGDSG VYQCLSEERV KNKTVFQVVA KHVLEVKVVP KPVVAPTLSV
   661  VQTEGSRIAT KVLVASTQGS SPPTPAVQAT SSGAITLPPK PAPTGTSCEP KIVINTVPQL
   721  HSEKTMYLKS SDNRLLMSLF LFFFVLFLCL FFYNCYKGYL PRQCLKFRSA LLIGKKKPKS
   781  DFCDREQSLK ETLVEPGSFS QQNGEHPKPA LDTGYETEQD TITSKVPTDR EDSQRIDDLS
   841  ARDKPFDVKC ELKFADSDAD GD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SEMA4D can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.36
Highest tissue expression
326 nTPM

Expression across tissuesHPA

Tissue

  • spinal cord: 326 nTPM
  • midbrain: 124 nTPM
  • skeletal muscle: 83 nTPM
  • hippocampal formation: 78 nTPM
  • basal ganglia: 60 nTPM
  • lymph node: 58 nTPM

Single-cell type

  • neutrophils: 757 nCPM
  • oligodendrocytes: 483 nCPM
  • esophageal apical cells: 270 nCPM
  • t-cells: 239 nCPM
  • microglia: 235 nCPM
  • platelets: 220 nCPM

Immune cell

  • eosinophil: 38 nTPM
  • neutrophil: 37 nTPM
  • naive CD8 T-cell: 23 nTPM
  • gdT-cell: 22 nTPM
  • naive CD4 T-cell: 20 nTPM
  • memory CD8 T-cell: 19 nTPM

Brain region

  • white matter: 490 nTPM
  • medulla oblongata: 313 nTPM
  • pons: 276 nTPM
  • basal ganglia: 273 nTPM
  • midbrain: 270 nTPM
  • cerebellum: 237 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.31
gnomAD pLI
0.97
gnomAD missense Z
1
DepMap mean gene effect
-0.02
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SEMA4D in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SEMA4D as an antibody target. Whether an autoantibody or antibody against SEMA4D could matter depends on whether native SEMA4D is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SEMA4D is annotated at the cell surface, where native SEMA4D is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label SEMA4D as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/SEMA4D. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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