Seroatlas · Human Serome Atlas

RPP25L

Ribonuclease P protein subunit p25-like protein

Also known as: bA296L22.5, C9orf23, MGC29635, RP25L_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8N5L8
Gene: RPP25L
Ensembl: ENSG00000164967
Chromosome: 9
Canonical length: 163 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nucleoli,Cytosol

OverviewNCBI Gene

This gene encodes a protein that appears to belong to a family of evolutionarily related proteins (DUF78), that may share one or more domains in common. Members of this family are small archaebacterial proteins with no known function. Alternative splicing has been observed at this locus and two variants, both encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

163 residues, UniProt reviewed canonical sequence.

>Q8N5L8|RPP25L
     1  MEHYRKAGSV ELPAPSPMPQ LPPDTLEMRV RDGSKIRNLL GLALGRLEGG SARHVVFSGS
    61  GRAAGKAVSC AEIVKRRVPG LHQLTKLRFL QTEDSWVPAS PDTGLDPLTV RRHVPAVWVL
   121  LSRDPLDPNE CGYQPPGAPP GLGSMPSSSC GPRSRRRARD TRS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RPP25L can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.39
Highest tissue expression: 116 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 116 nTPM
tongue: 96 nTPM
liver: 51 nTPM
heart muscle: 45 nTPM
basal ganglia: 32 nTPM
kidney: 32 nTPM

Single-cell type

enterocytes: 121 nCPM
hepatocytes: 88 nCPM
esophageal basal cells: 77 nCPM
esophageal suprabasal cells: 75 nCPM
cytotrophoblasts: 72 nCPM
migrating cytotrophoblasts: 67 nCPM

Immune cell

basophil: 72 nTPM
T-reg: 69 nTPM
naive B-cell: 68 nTPM
non-classical monocyte: 58 nTPM
memory B-cell: 57 nTPM
NK-cell: 57 nTPM

Brain region

thalamus: 24 nTPM
cerebellum: 23 nTPM
cerebral cortex: 22 nTPM
pons: 22 nTPM
basal ganglia: 20 nTPM
hypothalamus: 20 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.81
gnomAD pLI: 0.68
gnomAD missense Z: 0.79
DepMap mean gene effect: -0.4
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

tRNA 5'-leader removal

Molecular functions

RNA binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Nucleus

InteractionsUniProt · HPA

Protein binding partners of RPP25L in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RPP25L as an antibody target. Whether an autoantibody or antibody against RPP25L could matter depends on whether native RPP25L is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RPP25L is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RPP25L as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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