Seroatlas · Human Serome Atlas

RNF114

E3 ubiquitin-protein ligase RNF114

Also known as: PSORS12, RN114_HUMAN, ZNF313

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9Y508
Gene
RNF114
Ensembl
ENSG00000124226
Chromosome
20
Canonical length
228 aa
Protein class
Enzymes, Predicted intracellular proteins
Subcellular location
Plasma membrane,Cytosol

OverviewNCBI Gene

Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in protein polyubiquitination and ubiquitin-dependent protein catabolic process. Located in cytosol and plasma membrane. Biomarker of male infertility. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

228 residues, UniProt reviewed canonical sequence.

>Q9Y508|RNF114
     1  MAAQQRDCGG AAQLAGPAAE ADPLGRFTCP VCLEVYEKPV QVPCGHVFCS ACLQECLKPK
    61  KPVCGVCRSA LAPGVRAVEL ERQIESTETS CHGCRKNFFL SKIRSHVATC SKYQNYIMEG
   121  VKATIKDASL QPRNVPNRYT FPCPYCPEKN FDQEGLVEHC KLFHSTDTKS VVCPICASMP
   181  WGDPNYRSAN FREHIQRRHR FSYDTFVDYD VDEEDMMNQV LQRSIIDQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RNF114 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.41
Highest tissue expression
154 nTPM

Expression across tissuesHPA

Tissue

  • testis: 154 nTPM
  • skeletal muscle: 76 nTPM
  • spleen: 50 nTPM
  • pancreas: 49 nTPM
  • tongue: 49 nTPM
  • bone marrow: 49 nTPM

Single-cell type

  • late primary spermatocytes: 1,602 nCPM
  • early spermatids: 1,419 nCPM
  • late spermatids: 1,104 nCPM
  • oocytes: 636 nCPM
  • gastric progenitor cells: 187 nCPM
  • syncytiotrophoblasts: 167 nCPM

Immune cell

  • non-classical monocyte: 113 nTPM
  • basophil: 113 nTPM
  • neutrophil: 101 nTPM
  • classical monocyte: 98 nTPM
  • total PBMC: 93 nTPM
  • intermediate monocyte: 93 nTPM

Brain region

  • white matter: 42 nTPM
  • basal ganglia: 39 nTPM
  • thalamus: 39 nTPM
  • spinal cord: 38 nTPM
  • medulla oblongata: 38 nTPM
  • cerebellum: 36 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.8
gnomAD pLI
0.05
gnomAD missense Z
1.03
DepMap mean gene effect
-0.13
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 15% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RNF114 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RNF114 as an antibody target. Whether an autoantibody or antibody against RNF114 could matter depends on whether native RNF114 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RNF114 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RNF114 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RNF114. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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