RFX8

Protein identityUniProt · HPA

UniProt accession

Q6ZV50

Gene

RFX8

Ensembl

ENSG00000196460

Chromosome

2

Canonical length

586 aa

Protein class

Predicted intracellular proteins, Transcription factors

Subcellular location

Nucleoplasm,Nuclear bodies

OverviewNCBI Gene

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be located in chromatin. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

586 residues, UniProt reviewed canonical sequence.

>Q6ZV50|RFX8
     1  MAEGVPASPS SGEGSRGPHS GVIQWLVDNF CICEECSVPR CLMYEIYVET CGQNTENQVN
    61  PATFGKLVRL VFPDLGTRRL GTRGSARYHY DGICIKKSSF FYAQYCYLIG EKRYHSGDAI
   121  AFEKSTNYNS IIQQEATCED HSPMKTDPVG SPLSEFRRCP FLEQEQAKKY SCNMMAFLAD
   181  EYCNYCRDIL RNVEDLLTSF WKSLQQDTVM LMSLPDVCQL FKCYDVQLYK GIEDVLLHDF
   241  LEDVSIQYLK SVQLFSKKFK LWLLNALEGV PALLQISKLK EVTLFVKRLR RKTYLSNMAK
   301  TMRMVLKSKR RVSVLKSDLQ AIINQGTLAT SKKALASDRS GADELENNPE MKCLRNLISL
   361  LGTSTDLRVF LSCLSSHLQA FVFQTSRSKE EFTKLAASFQ LRWNLLLTAV SKAMTLCHRD
   421  SFGSWHLFHL LLLEYMIHIL QSCLEEEEEE EDMGTVKEML PDDPTLGQPD QALFHSLNSS
   481  LSQACASPSM EPLGVMPTHM GQGRYPVGVS NMVLRILGFL VDTAMGNKLI QVLLEDETTE
   541  SAVKLSLPMG QEALITLKDG QQFVIQISDV PQSSEDIYFR ENNANV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RFX8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.39

Highest tissue expression

3.5 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.09

gnomAD pLI

0

gnomAD missense Z

1.25

DepMap mean gene effect

-0.1

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• regulation of transcription by RNA polymerase II

Molecular functions

• DNA-binding transcription factor activity, RNA polymerase II-specific
• RNA polymerase II cis-regulatory region sequence-specific DNA binding

Cellular components

• chromatin
• nucleus

Protein domainsUniProt · Pfam · InterPro

• DNA-binding RFX-type winged-helix domain
• Winged helix-like DNA-binding domain superfamily
• Winged helix DNA-binding domain superfamily
• RFX-like DNA-binding protein
• RFX1-4/6/8-like, BCD domain
• RFX DNA-binding domain
• RFX BCD domain

KeywordsUniProt

• DNA-binding
• Nucleus
• Transcription
• Transcription regulation