Seroatlas · Human Serome Atlas

RFFL

E3 ubiquitin-protein ligase rififylin

Also known as: CARP-2, CARP2, fring, RFFL_HUMAN, rififylin, RNF189, RNF34L

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8WZ73
Gene: RFFL
Ensembl: ENSG00000092871
Chromosome: 17
Canonical length: 363 aa
Protein class: Enzymes, Predicted intracellular proteins
Subcellular location: Vesicles

OverviewNCBI Gene

Enables enzyme binding activity; p53 binding activity; and ubiquitin protein ligase activity. Involved in negative regulation of signal transduction; proteasome-mediated ubiquitin-dependent protein catabolic process; and protein K48-linked ubiquitination. Located in endosome membrane and plasma membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

363 residues, UniProt reviewed canonical sequence.

>Q8WZ73|RFFL
     1  MWATCCNWFC LDGQPEEVPP PQGARMQAYS NPGYSSFPSP TGLEPSCKSC GAHFANTARK
    61  QTCLDCKKNF CMTCSSQVGN GPRLCLLCQR FRATAFQREE LMKMKVKDLR DYLSLHDIST
   121  EMCREKEELV LLVLGQQPVI SQEDRTRAST LSPDFPEQQA FLTQPHSSMV PPTSPNLPSS
   181  SAQATSVPPA QVQENQQANG HVSQDQEEPV YLESVARVPA EDETQSIDSE DSFVPGRRAS
   241  LSDLTDLEDI EGLTVRQLKE ILARNFVNYK GCCEKWELME RVTRLYKDQK GLQHLVSGAE
   301  DQNGGAVPSG LEENLCKICM DSPIDCVLLE CGHMVTCTKC GKRMNECPIC RQYVIRAVHV
   361  FRS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RFFL can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.49
Highest tissue expression: 55 nTPM

Expression across tissuesHPA

Tissue

spinal cord: 55 nTPM
liver: 45 nTPM
bone marrow: 39 nTPM
thyroid gland: 37 nTPM
esophagus: 34 nTPM
midbrain: 28 nTPM

Single-cell type

late spermatids: 152 nCPM
oligodendrocytes: 106 nCPM
esophageal apical cells: 85 nCPM
neutrophils: 75 nCPM
early spermatids: 46 nCPM
hepatocytes: 42 nCPM

Immune cell

neutrophil: 56 nTPM
eosinophil: 34 nTPM
T-reg: 30 nTPM
basophil: 29 nTPM
MAIT T-cell: 21 nTPM
memory CD4 T-cell: 19 nTPM

Brain region

white matter: 203 nTPM
medulla oblongata: 172 nTPM
basal ganglia: 137 nTPM
pons: 129 nTPM
midbrain: 126 nTPM
thalamus: 124 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.5
gnomAD pLI: 0.41
gnomAD missense Z: 1.4
DepMap mean gene effect: -0.1
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RFFL in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RFFL as an antibody target. Whether an autoantibody or antibody against RFFL could matter depends on whether native RFFL is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RFFL is annotated at the cell surface, where native RFFL is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label RFFL as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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