Seroatlas · Human Serome Atlas

RAPH1

Ras-associated and pleckstrin homology domains-containing protein 1

Also known as: ALS2CR18, ALS2CR9, KIAA1681, RAPH1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q70E73
Gene
RAPH1
Ensembl
ENSG00000173166
Chromosome
2
Canonical length
1250 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nuclear bodies,Plasma membrane,Cytosol

OverviewNCBI Gene

This gene encodes a protein that belongs to the Mig10/Rap1-interacting adaptor molecule/Lamellipodin family of adapter proteins, which function in cell migration. Members of this family contain pleckstrin-homology domains, Ras-association domains, and proline-rich C-termini. The protein encoded by this gene regulates actin dynamics through interaction with Ena/Vasodilator proteins as well as direct binding to filamentous actin to regulate actin network assembly. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016]

Canonical amino-acid sequenceUniProt

1250 residues, UniProt reviewed canonical sequence.

>Q70E73|RAPH1
     1  MEQLSDEEID HGAEEDSDKE DQDLDKMFGA WLGELDKLTQ SLDSDKPMEP VKRSPLRQET
    61  NMANFSYRFS IYNLNEALNQ GETVDLDALM ADLCSIEQEL SSIGSGNSKR QITETKATQK
   121  LPVSRHTLKH GTLKGLSSSS NRIAKPSHAS YSLDDVTAQL EQASLSMDEA AQQSVLEDTK
   181  PLVTNQHRRT ASAGTVSDAE VHSISNSSHS SITSAASSMD SLDIDKVTRP QELDLTHQGQ
   241  PITEEEQAAK LKAEKIRVAL EKIKEAQVKK LVIRVHMSDD SSKTMMVDER QTVRQVLDNL
   301  MDKSHCGYSL DWSLVETVSE LQMERIFEDH ENLVENLLNW TRDSQNKLIF MERIEKYALF
   361  KNPQNYLLGK KETAEMADRN KEVLLEECFC GSSVTVPEIE GVLWLKDDGK KSWKKRYFLL
   421  RASGIYYVPK GKAKVSRDLV CFLQLDHVNV YYGQDYRNKY KAPTDYCLVL KHPQIQKKSQ
   481  YIKYLCCDDV RTLHQWVNGI RIAKYGKQLY MNYQEALKRT ESAYDWTSLS SSSIKSGSSS
   541  SSIPESQSNH SNQSDSGVSD TQPAGHVRSQ SIVSSVFSEA WKRGTQLEES SKARMESMNR
   601  PYTSLVPPLS PQPKIVTPYT ASQPSPPLPP PPPPPPPPPP PPPPPPPPLP SQSAPSAGSA
   661  APMFVKYSTI TRLQNASQHS GALFKPPTPP VMQSQSVKPQ ILVPPNGVVP PPPPPPPPPT
   721  PGSAMAQLKP APCAPSLPQF SAPPPPLKIH QVQHITQVAP PTPPPPPPIP APLPPQAPPK
   781  PLVTIPAPTS TKTVAPVVTQ AAPPTPTPPV PPAKKQPAFP ASYIPPSPPT PPVPVPPPTL
   841  PKQQSFCAKP PPSPLSPVPS VVKQIASQFP PPPTPPAMES QPLKPVPANV APQSPPAVKA
   901  KPKWQPSSIP VPSPDFPPPP PESSLVFPPP PPSPVPAPPP PPPPTASPTP DKSGSPGKKT
   961  SKTSSPGGKK PPPTPQRNSS IKSSSGAEHP EPKRPSVDSL VSKFTPPAES GSPSKETLPP
  1021  PAAPPKPGKL NLSGVNLPGV LQQGCVSAKA PVLSGRGKDS VVEFPSPPSD SDFPPPPPET
  1081  ELPLPPIEIP AVFSGNTSPK VAVVNPQPQQ WSKMSVKKAP PPTRPKRNDS TRLTQAEISE
  1141  QPTMATVVPQ VPTSPKSSLS VQPGFLADLN RTLQRKSITR HGSLSSRMSR AEPTATMDDM
  1201  ALPPPPPELL SDQQKAGYGG SHISGYATLR RGPPPAPPKR DQNTKLSRDW

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RAPH1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.6
Highest tissue expression
58 nTPM

Expression across tissuesHPA

Tissue

  • liver: 58 nTPM
  • thyroid gland: 28 nTPM
  • spleen: 16 nTPM
  • lung: 13 nTPM
  • breast: 13 nTPM
  • placenta: 12 nTPM

Single-cell type

  • cardiomyocytes: 392 nCPM
  • epicardial cells: 384 nCPM
  • thymic myoid cells: 294 nCPM
  • endometrial luminal cells: 268 nCPM
  • bergmann glia: 264 nCPM
  • epididymal basal cells: 261 nCPM

Immune cell

  • intermediate monocyte: 0.7 nTPM
  • non-classical monocyte: 0.7 nTPM
  • myeloid DC: 0.3 nTPM
  • classical monocyte: 0.2 nTPM
  • NK-cell: 0.2 nTPM
  • MAIT T-cell: 0.1 nTPM

Brain region

  • hypothalamus: 46 nTPM
  • cerebral cortex: 44 nTPM
  • midbrain: 44 nTPM
  • pons: 37 nTPM
  • hippocampal formation: 37 nTPM
  • basal ganglia: 36 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.22
gnomAD pLI
1
gnomAD missense Z
1.39
DepMap mean gene effect
-0.13
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RAPH1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RAPH1 as an antibody target. Whether an autoantibody or antibody against RAPH1 could matter depends on whether native RAPH1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RAPH1 is annotated at the cell surface, where native RAPH1 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label RAPH1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RAPH1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...