PURB

Protein identityUniProt · HPA

UniProt accession

Q96QR8

Gene

PURB

Ensembl

ENSG00000146676

Chromosome

7

Canonical length

312 aa

Protein class

Predicted intracellular proteins, Transcription factors

Subcellular location

Nucleoplasm

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

312 residues, UniProt reviewed canonical sequence.

>Q96QR8|PURB
     1  MADGDSGSER GGGGGPCGFQ PASRGGGEQE TQELASKRLD IQNKRFYLDV KQNAKGRFLK
    61  IAEVGAGGSK SRLTLSMAVA AEFRDSLGDF IEHYAQLGPS SPEQLAAGAE EGGGPRRALK
   121  SEFLVRENRK YYLDLKENQR GRFLRIRQTV NRGGGGFGAG PGPGGLQSGQ TIALPAQGLI
   181  EFRDALAKLI DDYGGEDDEL AGGPGGGAGG PGGGLYGELP EGTSITVDSK RFFFDVGCNK
   241  YGVFLRVSEV KPSYRNAITV PFKAWGKFGG AFCRYADEMK EIQERQRDKL YERRGGGSGG
   301  GEESEGEEVD ED

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PURB can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.45

Highest tissue expression

23 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.64

gnomAD pLI

0.63

gnomAD missense Z

1.74

DepMap mean gene effect

-0.01

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 12% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• negative regulation of transcription by RNA polymerase II
• positive regulation of transcription by RNA polymerase II
• regulation of myeloid cell differentiation
• regulation of transcription by RNA polymerase II

Molecular functions

• DNA-binding transcription factor activity, RNA polymerase II-specific
• DNA-binding transcription factor binding
• DNA-binding transcription repressor activity, RNA polymerase II-specific
• mRNA binding
• mRNA regulatory element binding translation repressor activity
• purine-rich negative regulatory element binding
• RNA binding
• RNA polymerase II transcription regulatory region sequence-specific DNA binding
• single-stranded DNA binding
• SMAD binding

Cellular components

• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Purine-rich element binding protein family
• PurA ssDNA and RNA-binding protein

KeywordsUniProt

• Acetylation
• Activator
• DNA-binding
• Methylation
• Nucleus
• Phosphoprotein
• Repressor
• Transcription
• Transcription regulation

InteractionsUniProt · HPA

Protein binding partners of PURB in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MYOCD
• SRF
• YBX1
• PURG