Seroatlas · Human Serome Atlas

PPP1R16A

Protein phosphatase 1 regulatory subunit 16A

Also known as: MGC14333, MYPT3, PP16A_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q96I34
Gene
PPP1R16A
Ensembl
ENSG00000160972
Chromosome
8
Canonical length
528 aa
Protein class
Predicted intracellular proteins
Subcellular location
Plasma membrane

OverviewNCBI Gene

Myosin light chain kinase and phosphatase (MLCP) complexes control the phosphorylation states of regulatory myosin light chains, which is crucial for muscle and intracellular movement. MLCPs typically contain a catalytic protein phosphatase 1 (PP1c) subunit, a myosin phosphatase targeting (MYPT) subunit, and another smaller subunit. The protein encoded by this gene represents an MYPT subunit, which is responsible for directing PP1c to its intended targets. However, while the phosphorylation of other MYPT members results in PP1c inactivation, phosphorylation of the encoded protein by protein kinase A results in PP1c activation. [provided by RefSeq, Jan 2020]

Canonical amino-acid sequenceUniProt

528 residues, UniProt reviewed canonical sequence.

>Q96I34|PPP1R16A
     1  MAEHLELLAE MPMVGRMSTQ ERLKHAQKRR AQQVKMWAQA EKEAQGKKGP GERPRKEAAS
    61  QGLLKQVLFP PSVVLLEAAA RNDLEEVRQF LGSGVSPDLA NEDGLTALHQ CCIDDFREMV
   121  QQLLEAGANI NACDSECWTP LHAAATCGHL HLVELLIASG ANLLAVNTDG NMPYDLCDDE
   181  QTLDCLETAM ADRGITQDSI EAARAVPELR MLDDIRSRLQ AGADLHAPLD HGATLLHVAA
   241  ANGFSEAAAL LLEHRASLSA KDQDGWEPLH AAAYWGQVPL VELLVAHGAD LNAKSLMDET
   301  PLDVCGDEEV RAKLLELKHK HDALLRAQSR QRSLLRRRTS SAGSRGKVVR RVSLTQRTDL
   361  YRKQHAQEAI VWQQPPPTSP EPPEDNDDRQ TGAELRPPPP EEDNPEVVRP HNGRVGGSPV
   421  RHLYSKRLDR SVSYQLSPLD STTPHTLVHD KAHHTLADLK RQRAAAKLQR PPPEGPESPE
   481  TAEPGLPGDT VTPQPDCGFR AGGDPPLLKL TAPAVEAPVE RRPCCLLM

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PPP1R16A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.44
Highest tissue expression
35 nTPM

Expression across tissuesHPA

Tissue

  • pituitary gland: 35 nTPM
  • testis: 31 nTPM
  • hippocampal formation: 26 nTPM
  • thyroid gland: 26 nTPM
  • kidney: 26 nTPM
  • duodenum: 25 nTPM

Single-cell type

  • enterocytes: 393 nCPM
  • breast lactating cells: 263 nCPM
  • hepatocytes: 239 nCPM
  • parietal cells: 233 nCPM
  • cholangiocytes: 179 nCPM
  • gastric chief cells: 130 nCPM

Immune cell

  • non-classical monocyte: 1.6 nTPM
  • intermediate monocyte: 1.2 nTPM
  • myeloid DC: 1.1 nTPM
  • gdT-cell: 1 nTPM
  • MAIT T-cell: 0.9 nTPM
  • memory CD8 T-cell: 0.9 nTPM

Brain region

  • choroid plexus: 40 nTPM
  • cerebral cortex: 32 nTPM
  • hippocampal formation: 32 nTPM
  • midbrain: 28 nTPM
  • medulla oblongata: 27 nTPM
  • thalamus: 27 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.65
gnomAD pLI
0
gnomAD missense Z
0.13
DepMap mean gene effect
0.01
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 13% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PPP1R16A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PPP1R16A as an antibody target. Whether an autoantibody or antibody against PPP1R16A could matter depends on whether native PPP1R16A is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PPP1R16A is annotated at the cell surface, where native PPP1R16A is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label PPP1R16A as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/PPP1R16A. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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