Seroatlas · Human Serome Atlas

POLI

DNA polymerase iota

Also known as: POLI_HUMAN, RAD30B, RAD3OB

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9UNA4
Gene: POLI
Ensembl: ENSG00000101751
Chromosome: 18
Canonical length: 740 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Nuclear speckles,Cytoplasmic bodies

OverviewNCBI Gene

The protein encoded by this gene is an error-prone DNA polymerase involved in DNA repair. The encoded protein promotes DNA synthesis across lesions in the template DNA, which other polymerases cannot do. The encoded polymerase inserts deoxynucleotides across lesions and then relies on DNA polymerase zeta to extend the nascent DNA strand to bypass the lesion. [provided by RefSeq, May 2017]

Canonical amino-acid sequenceUniProt

740 residues, UniProt reviewed canonical sequence.

>Q9UNA4|POLI
     1  MEKLGVEPEE EGGGDDDEED AEAWAMELAD VGAAASSQGV HDQVLPTPNA SSRVIVHVDL
    61  DCFYAQVEMI SNPELKDKPL GVQQKYLVVT CNYEARKLGV KKLMNVRDAK EKCPQLVLVN
   121  GEDLTRYREM SYKVTELLEE FSPVVERLGF DENFVDLTEM VEKRLQQLQS DELSAVTVSG
   181  HVYNNQSINL LDVLHIRLLV GSQIAAEMRE AMYNQLGLTG CAGVASNKLL AKLVSGVFKP
   241  NQQTVLLPES CQHLIHSLNH IKEIPGIGYK TAKCLEALGI NSVRDLQTFS PKILEKELGI
   301  SVAQRIQKLS FGEDNSPVIL SGPPQSFSEE DSFKKCSSEV EAKNKIEELL ASLLNRVCQD
   361  GRKPHTVRLI IRRYSSEKHY GRESRQCPIP SHVIQKLGTG NYDVMTPMVD ILMKLFRNMV
   421  NVKMPFHLTL LSVCFCNLKA LNTAKKGLID YYLMPSLSTT SRSGKHSFKM KDTHMEDFPK
   481  DKETNRDFLP SGRIESTRTR ESPLDTTNFS KEKDINEFPL CSLPEGVDQE VFKQLPVDIQ
   541  EEILSGKSRE KFQGKGSVSC PLHASRGVLS FFSKKQMQDI PINPRDHLSS SKQVSSVSPC
   601  EPGTSGFNSS SSSYMSSQKD YSYYLDNRLK DERISQGPKE PQGFHFTNSN PAVSAFHSFP
   661  NLQSEQLFSR NHTTDSHKQT VATDSHEGLT ENREPDSVDE KITFPSDIDP QVFYELPEAV
   721  QKELLAEWKR AGSDFHIGHK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against POLI can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.46
Highest tissue expression: 36 nTPM

Expression across tissuesHPA

Tissue

testis: 36 nTPM
thyroid gland: 29 nTPM
retina: 24 nTPM
adipose tissue: 20 nTPM
breast: 17 nTPM
parathyroid gland: 15 nTPM

Single-cell type

late primary spermatocytes: 149 nCPM
early spermatids: 93 nCPM
sertoli cells: 93 nCPM
podocytes: 84 nCPM
adipocytes: 75 nCPM
early primary spermatocytes: 70 nCPM

Immune cell

naive CD4 T-cell: 7 nTPM
eosinophil: 6.7 nTPM
naive B-cell: 6.6 nTPM
classical monocyte: 6.4 nTPM
myeloid DC: 6.1 nTPM
T-reg: 6.1 nTPM

Brain region

white matter: 30 nTPM
basal ganglia: 26 nTPM
medulla oblongata: 24 nTPM
cerebral cortex: 24 nTPM
spinal cord: 23 nTPM
thalamus: 23 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.93
gnomAD pLI: 0
gnomAD missense Z: -0.51
DepMap mean gene effect: 0.12
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of POLI in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads POLI as an antibody target. Whether an autoantibody or antibody against POLI could matter depends on whether native POLI is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

POLI is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label POLI as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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