Seroatlas · Human Serome Atlas

PDE8A

High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A

Also known as: HsT19550, PDE8A_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O60658
Gene: PDE8A
Ensembl: ENSG00000073417
Chromosome: 15
Canonical length: 829 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Golgi apparatus

OverviewNCBI Gene

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Canonical amino-acid sequenceUniProt

829 residues, UniProt reviewed canonical sequence.

>O60658|PDE8A
     1  MGCAPSIHIS ERLVAEDAPS PAAPPLSSGG PRLPQGQKTA ALPRTRGAGL LESELRDGSG
    61  KKVAVADVQF GPMRFHQDQL QVLLVFTKED NQCNGFCRAC EKAGFKCTVT KEAQAVLACF
   121  LDKHHDIIII DHRNPRQLDA EALCRSIRSS KLSENTVIVG VVRRVDREEL SVMPFISAGF
   181  TRRYVENPNI MACYNELLQL EFGEVRSQLK LRACNSVFTA LENSEDAIEI TSEDRFIQYA
   241  NPAFETTMGY QSGELIGKEL GEVPINEKKA DLLDTINSCI RIGKEWQGIY YAKKKNGDNI
   301  QQNVKIIPVI GQGGKIRHYV SIIRVCNGNN KAEKISECVQ SDTHTDNQTG KHKDRRKGSL
   361  DVKAVASRAT EVSSQRRHSS MARIHSMTIE APITKVINII NAAQESSPMP VTEALDRVLE
   421  ILRTTELYSP QFGAKDDDPH ANDLVGGLMS DGLRRLSGNE YVLSTKNTQM VSSNIITPIS
   481  LDDVPPRIAR AMENEEYWDF DIFELEAATH NRPLIYLGLK MFARFGICEF LHCSESTLRS
   541  WLQIIEANYH SSNPYHNSTH SADVLHATAY FLSKERIKET LDPIDEVAAL IAATIHDVDH
   601  PGRTNSFLCN AGSELAILYN DTAVLESHHA ALAFQLTTGD DKCNIFKNME RNDYRTLRQG
   661  IIDMVLATEM TKHFEHVNKF VNSINKPLAT LEENGETDKN QEVINTMLRT PENRTLIKRM
   721  LIKCADVSNP CRPLQYCIEW AARISEEYFS QTDEEKQQGL PVVMPVFDRN TCSIPKSQIS
   781  FIDYFITDMF DAWDAFVDLP DLMQHLDNNF KYWKGLDEMK LRNLRPPPE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PDE8A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.34
Highest tissue expression: 28 nTPM

Expression across tissuesHPA

Tissue

liver: 28 nTPM
kidney: 23 nTPM
colon: 23 nTPM
rectum: 21 nTPM
urinary bladder: 17 nTPM
parathyroid gland: 16 nTPM

Single-cell type

oligodendrocytes: 1,223 nCPM
proximal tubule cells: 657 nCPM
mast cells: 562 nCPM
distal convoluted tubule cells: 491 nCPM
adrenal cortex cells: 457 nCPM
urothelial cells: 457 nCPM

Immune cell

non-classical monocyte: 1.6 nTPM
T-reg: 1.1 nTPM
classical monocyte: 0.9 nTPM
intermediate monocyte: 0.7 nTPM
naive CD4 T-cell: 0.7 nTPM
eosinophil: 0.6 nTPM

Brain region

white matter: 85 nTPM
basal ganglia: 60 nTPM
midbrain: 53 nTPM
thalamus: 50 nTPM
medulla oblongata: 44 nTPM
cerebral cortex: 43 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.53
gnomAD pLI: 0
gnomAD missense Z: 1.1
DepMap mean gene effect: 0
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PDE8A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

RAF1

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PDE8A as an antibody target. Whether an autoantibody or antibody against PDE8A could matter depends on whether native PDE8A is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PDE8A is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PDE8A as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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