PAPOLA

Protein identityUniProt · HPA

UniProt accession

P51003

Gene

PAPOLA

Ensembl

ENSG00000090060

Chromosome

14

Canonical length

745 aa

Protein class

Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins, Predicted membrane proteins

Subcellular location

Nucleoplasm

OverviewNCBI Gene

The protein encoded by this gene belongs to the poly(A) polymerase family. It is required for the addition of adenosine residues for the creation of the 3'-poly(A) tail of mRNAs. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Canonical amino-acid sequenceUniProt

745 residues, UniProt reviewed canonical sequence.

>P51003|PAPOLA
     1  MPFPVTTQGS QQTQPPQKHY GITSPISLAA PKETDCVLTQ KLIETLKPFG VFEEEEELQR
    61  RILILGKLNN LVKEWIREIS ESKNLPQSVI ENVGGKIFTF GSYRLGVHTK GADIDALCVA
   121  PRHVDRSDFF TSFYDKLKLQ EEVKDLRAVE EAFVPVIKLC FDGIEIDILF ARLALQTIPE
   181  DLDLRDDSLL KNLDIRCIRS LNGCRVTDEI LHLVPNIDNF RLTLRAIKLW AKRHNIYSNI
   241  LGFLGGVSWA MLVARTCQLY PNAIASTLVH KFFLVFSKWE WPNPVLLKQP EECNLNLPVW
   301  DPRVNPSDRY HLMPIITPAY PQQNSTYNVS VSTRMVMVEE FKQGLAITDE ILLSKAEWSK
   361  LFEAPNFFQK YKHYIVLLAS APTEKQRLEW VGLVESKIRI LVGSLEKNEF ITLAHVNPQS
   421  FPAPKENPDK EEFRTMWVIG LVFKKTENSE NLSVDLTYDI QSFTDTVYRQ AINSKMFEVD
   481  MKIAAMHVKR KQLHQLLPNH VLQKKKKHST EGVKLTALND SSLDLSMDSD NSMSVPSPTS
   541  ATKTSPLNSS GSSQGRNSPA PAVTAASVTN IQATEVSVPQ VNSSESSGGT SSESIPQTAT
   601  QPAISPPPKP TVSRVVSSTR LVNPPPRSSG NAATSGNAAT KIPTPIVGVK RTSSPHKEES
   661  PKKTKTEEDE TSEDANCLAL SGHDKTEAKE QLDTETSTTQ SETIQTAASL LASQKTSSTD
   721  LSDIPALPAN PIPVIKNSIK LRLNR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PAPOLA can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.4

Highest tissue expression

91 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.18

gnomAD pLI

1

gnomAD missense Z

3.55

DepMap mean gene effect

-0.18

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway
• cytosolic mRNA polyadenylation
• mRNA 3'-end processing

Molecular functions

• ATP binding
• magnesium ion binding
• manganese ion binding
• poly(A) RNA polymerase activity
• RNA binding

Cellular components

• cytoplasm
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Poly(A) polymerase, RNA-binding domain
• Poly(A) polymerase, central domain
• Nucleotidyltransferase, class I-like, C-terminal
• Poly(A) polymerase
• Nucleotidyltransferase superfamily
• Poly(A) polymerase, nucleotidyltransferase domain
• Poly(A) polymerase predicted RNA binding domain
• Poly(A) polymerase central domain
• Poly(A) polymerase nucleotidyltransferase domain

KeywordsUniProt

• Acetylation
• ATP-binding
• Cytoplasm
• Isopeptide bond
• Magnesium
• Manganese
• Metal-binding
• mRNA processing
• Nucleotide-binding
• Nucleus
• Phosphoprotein
• RNA-binding
• Transferase
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of PAPOLA in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• AHCYL1
• FIP1L1
• KPNB1
• NUDT21
• PABPN1