Seroatlas · Human Serome Atlas

NUP35

Nucleoporin NUP35

Also known as: MP44, NUP35_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8NFH5
Gene
NUP35
Ensembl
ENSG00000163002
Chromosome
2
Canonical length
326 aa
Protein class
Metabolic proteins, Predicted intracellular proteins, Transporters
Subcellular location
Nucleoplasm,Nuclear membrane,Plasma membrane

OverviewNCBI Gene

This gene encodes a member of the nucleoporin family. The encoded protein contains two membrane binding regions, is localized to the nuclear rim, and is part of the nuclear pore complex. All molecules entering or leaving the nucleus either diffuse through or are actively transported by the nuclear pore complex. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 7 and 10. [provided by RefSeq, Dec 2013]

Canonical amino-acid sequenceUniProt

326 residues, UniProt reviewed canonical sequence.

>Q8NFH5|NUP35
     1  MAAFAVEPQG PALGSEPMML GSPTSPKPGV NAQFLPGFLM GDLPAPVTPQ PRSISGPSVG
    61  VMEMRSPLLA GGSPPQPVVP AHKDKSGAPP VRSIYDDISS PGLGSTPLTS RRQPNISVMQ
   121  SPLVGVTSTP GTGQSMFSPA SIGQPRKTTL SPAQLDPFYT QGDSLTSEDH LDDSWVTVFG
   181  FPQASASYIL LQFAQYGNIL KHVMSNTGNW MHIRYQSKLQ ARKALSKDGR IFGESIMIGV
   241  KPCIDKSVME SSDRCALSSP SLAFTPPIKT LGTPTQPGST PRISTMRPLA TAYKASTSDY
   301  QVISDRQTPK KDESLVSKAM EYMFGW

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NUP35 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.6
Highest tissue expression
23 nTPM

Expression across tissuesHPA

Tissue

  • testis: 23 nTPM
  • liver: 13 nTPM
  • breast: 12 nTPM
  • tonsil: 10 nTPM
  • pancreas: 9.7 nTPM
  • thymus: 9.7 nTPM

Single-cell type

  • late spermatids: 769 nCPM
  • early spermatids: 207 nCPM
  • oocytes: 101 nCPM
  • late primary spermatocytes: 93 nCPM
  • erythrocyte progenitors: 55 nCPM
  • early primary spermatocytes: 41 nCPM

Immune cell

  • MAIT T-cell: 8.7 nTPM
  • naive CD4 T-cell: 7.5 nTPM
  • memory B-cell: 7.3 nTPM
  • naive CD8 T-cell: 6.7 nTPM
  • T-reg: 6.7 nTPM
  • naive B-cell: 6.6 nTPM

Brain region

  • cerebellum: 12 nTPM
  • basal ganglia: 8.9 nTPM
  • spinal cord: 7.3 nTPM
  • cerebral cortex: 7.2 nTPM
  • white matter: 6.8 nTPM
  • hippocampal formation: 6.1 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.66
gnomAD pLI
0.02
gnomAD missense Z
0.89
DepMap mean gene effect
-0.46
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NUP35 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NUP35 as an antibody target. Whether an autoantibody or antibody against NUP35 could matter depends on whether native NUP35 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NUP35 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NUP35 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NUP35. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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