NUCB2

Protein identityUniProt · HPA

UniProt accession

P80303

Gene

NUCB2

Ensembl

ENSG00000070081

Chromosome

11

Canonical length

420 aa

Protein class

Cancer-related genes, Plasma proteins, Predicted intracellular proteins, Predicted secreted proteins

Subcellular location

Golgi apparatus

Secretome location

Secreted to blood

OverviewNCBI Gene

This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011]

Canonical amino-acid sequenceUniProt

420 residues, UniProt reviewed canonical sequence.

>P80303|NUCB2
     1  MRWRTILLQY CFLLITCLLT ALEAVPIDID KTKVQNIHPV ESAKIEPPDT GLYYDEYLKQ
    61  VIDVLETDKH FREKLQKADI EEIKSGRLSK ELDLVSHHVR TKLDELKRQE VGRLRMLIKA
   121  KLDSLQDIGM DHQALLKQFD HLNHLNPDKF ESTDLDMLIK AATSDLEHYD KTRHEEFKKY
   181  EMMKEHERRE YLKTLNEEKR KEEESKFEEM KKKHENHPKV NHPGSKDQLK EVWEETDGLD
   241  PNDFDPKTFF KLHDVNSDGF LDEQELEALF TKELEKVYDP KNEEDDMVEM EEERLRMREH
   301  VMNEVDTNKD RLVTLEEFLK ATEKKEFLEP DSWETLDQQQ FFTEEELKEY ENIIALQENE
   361  LKKKADELQK QKEELQRQHD QLEAQKLEYH QVIQQMEQKK LQQGIPPSGP AGELKFEPHI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NUCB2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Secreted

Secreted

Yes

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.37

Highest tissue expression

478 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about NUCB2.

Disease | ImmuneIEDB

Conditions an epitope on NUCB2 was assayed in.

• type 1 diabetes mellitus T cell

ReferencesPubMed · IEDB

Publications for NUCB2 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.13

gnomAD pLI

0

gnomAD missense Z

0.9

DepMap mean gene effect

0.02

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• small GTPase-mediated signal transduction

Molecular functions

• calcium ion binding
• DNA binding
• G-protein alpha-subunit binding
• guanyl-nucleotide exchange factor activity

Cellular components

• cytosol
• endoplasmic reticulum
• endoplasmic reticulum-Golgi intermediate compartment
• extracellular exosome
• extracellular space
• Golgi apparatus
• nuclear outer membrane
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• EF-hand domain
• EF-hand domain pair
• EF-Hand 1, calcium-binding site
• Nucleobindin
• NUCB1-like, N-terminal domain
• EF-hand domain pair
• NUCB1-like, N-terminal domain

KeywordsUniProt

• Calcium
• Cleavage on pair of basic residues
• Cytoplasm
• DNA-binding
• Endoplasmic reticulum
• Golgi apparatus
• Guanine-nucleotide releasing factor
• Membrane
• Metal-binding
• Nucleus
• Phosphoprotein
• Repeat
• Secreted
• Signal

InteractionsUniProt · HPA

Protein binding partners of NUCB2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MC4R
• GADD45A
• NUCB1