Seroatlas · Human Serome Atlas

NSG1

Neuronal vesicle trafficking-associated protein 1

Also known as: D4S234E, NEEP21, NSG1_HUMAN, P21

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P42857
Gene
NSG1
Ensembl
ENSG00000168824
Chromosome
4
Canonical length
185 aa
Protein class
Predicted membrane proteins

OverviewNCBI Gene

Predicted to enable clathrin light chain binding activity. Involved in apoptotic process. Located in endoplasmic reticulum. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

185 residues, UniProt reviewed canonical sequence.

>P42857|NSG1
     1  MVKLGNNFAE KGTKQPLLED GFDTIPLMTP LDVNQLQFPP PDKVVVKTKT EYEPDRKKGK
    61  ARPPQIAEFT VSITEGVTER FKVSVLVLFA LAFLTCVVFL VVYKVYKYDR ACPDGFVLKN
   121  TQCIPEGLES YYAEQDSSAR EKFYTVINHY NLAKQSITRS VSPWMSVLSE EKLSEQETEA
   181  AEKSA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NSG1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.59
Highest tissue expression
133 nTPM

Expression across tissuesHPA

Tissue

  • pituitary gland: 133 nTPM
  • skin: 105 nTPM
  • cerebellum: 92 nTPM
  • hypothalamus: 91 nTPM
  • basal ganglia: 76 nTPM
  • cerebral cortex: 58 nTPM

Single-cell type

  • oocytes: 539 nCPM
  • melanocytes: 447 nCPM
  • retinal bipolar cells: 344 nCPM
  • epididymal efferent duct absorptive cells: 200 nCPM
  • corticotrophs: 173 nCPM
  • granulosa cells: 168 nCPM

Immune cell

  • MAIT T-cell: 59 nTPM
  • gdT-cell: 42 nTPM
  • memory CD4 T-cell: 38 nTPM
  • memory CD8 T-cell: 35 nTPM
  • naive CD8 T-cell: 26 nTPM
  • total PBMC: 13 nTPM

Brain region

  • hypothalamus: 242 nTPM
  • midbrain: 155 nTPM
  • cerebellum: 148 nTPM
  • pons: 144 nTPM
  • medulla oblongata: 142 nTPM
  • cerebral cortex: 126 nTPM

ReferencesPubMed · IEDB

Publications for NSG1 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Reference: AutoantibodyPubMed

2 publications

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.29
gnomAD pLI
0.01
gnomAD missense Z
-0.37
DepMap mean gene effect
0.14
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NSG1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NSG1 as an antibody target. Whether an autoantibody or antibody against NSG1 could matter depends on whether native NSG1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NSG1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NSG1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NSG1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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