NQO1

Protein identityUniProt · HPA

UniProt accession

P15559

Gene

NQO1

Ensembl

ENSG00000181019

Chromosome

16

Canonical length

274 aa

Protein class

Cancer-related genes, Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Cytosol

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

274 residues, UniProt reviewed canonical sequence.

>P15559|NQO1
     1  MVGRRALIVL AHSERTSFNY AMKEAAAAAL KKKGWEVVES DLYAMNFNPI ISRKDITGKL
    61  KDPANFQYPA ESVLAYKEGH LSPDIVAEQK KLEAADLVIF QFPLQWFGVP AILKGWFERV
   121  FIGEFAYTYA AMYDKGPFRS KKAVLSITTG GSGSMYSLQG IHGDMNVILW PIQSGILHFC
   181  GFQVLEPQLT YSIGHTPADA RIQILEGWKK RLENIWDETP LYFAPSSLFD LNFQAGFLMK
   241  KEVQDEEKNK KFGLSVGHHL GKSIPTDNQI KARK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NQO1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.26

Highest tissue expression

493 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.51

gnomAD pLI

0

gnomAD missense Z

1.28

DepMap mean gene effect

0.02

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cell redox homeostasis
• cellular response to oxidative stress
• innate immune response
• negative regulation of ferroptosis
• negative regulation of protein catabolic process
• nitric oxide biosynthetic process
• protein catabolic process
• protein polyubiquitination
• removal of superoxide radicals
• response to caloric restriction
• response to lipopolysaccharide
• response to oxidative stress
• response to toxic substance
• synaptic transmission, cholinergic
• ubiquinone metabolic process
• vitamin E metabolic process
• vitamin K metabolic process
• xenobiotic metabolic process

Molecular functions

• cytochrome-b5 reductase activity, acting on NAD(P)H
• identical protein binding
• NAD(P)H dehydrogenase (quinone) activity
• NADH dehydrogenase (quinone) (non-electrogenic) activity
• NADPH dehydrogenase (quinone) activity
• RNA binding

Cellular components

• cytoplasm
• cytosol
• nucleus
• synapse

Protein domainsUniProt · Pfam · InterPro

• Flavodoxin-like fold
• Flavoprotein-like superfamily
• NAD(P)H dehydrogenase (quinone)
• Flavodoxin-like fold

KeywordsUniProt

• Cytoplasm
• FAD
• Flavoprotein
• Isopeptide bond
• NAD
• NADP
• Oxidoreductase
• Phosphoprotein
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of NQO1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• TP53
• TP73
• PDLIM4