NOVA1

Protein identityUniProt · HPA

UniProt accession

P51513

Gene

NOVA1

Ensembl

ENSG00000139910

Chromosome

14

Canonical length

507 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm,Nucleoli

OverviewNCBI Gene

This gene encodes a neuron-specific RNA-binding protein, a member of the Nova family of paraneoplastic disease antigens, that is recognized and inhibited by paraneoplastic antibodies. These antibodies are found in the sera of patients with paraneoplastic opsoclonus-ataxia, breast cancer, and small cell lung cancer. Alternatively spliced transcripts encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

507 residues, UniProt reviewed canonical sequence.

>P51513|NOVA1
     1  MMAAAPIQQN GTHTGVPIDL DPPDSRKRPL EAPPEAGSTK RTNTGEDGQY FLKVLIPSYA
    61  AGSIIGKGGQ TIVQLQKETG ATIKLSKSKD FYPGTTERVC LIQGTVEALN AVHGFIAEKI
   121  REMPQNVAKT EPVSILQPQT TVNPDRIKQT LPSSPTTTKS SPSDPMTTSR ANQVKIIVPN
   181  STAGLIIGKG GATVKAVMEQ SGAWVQLSQK PDGINLQERV VTVSGEPEQN RKAVELIIQK
   241  IQEDPQSGSC LNISYANVTG PVANSNPTGS PYANTAEVLP TAAAAAGLLG HANLAGVAAF
   301  PAVLSGFTGN DLVAITSALN TLASYGYNLN TLGLGLSQAA ATGALAAAAA SANPAAAAAN
   361  LLATYASEAS ASGSTAGGTA GTFALGSLAA ATAATNGYFG AASPLAASAI LGTEKSTDGS
   421  KDVVEIAVPE NLVGAILGKG GKTLVEYQEL TGARIQISKK GEFVPGTRNR KVTITGTPAA
   481  TQAAQYLITQ RITYEQGVRA ANPQKVG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NOVA1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.48

Highest tissue expression

64 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about NOVA1.

Disease | AutoantibodyPubMed

Conditions in which antibodies against NOVA1 are reported. Each links to that disease's full target list.

• Breast Neoplasms 16
• Paraneoplastic Syndromes, Nervous System 13
• Urticaria 5
• Adenocarcinoma 3
• Cerebellar Ataxia 3
• Limbic Encephalitis 3
• Lung Neoplasms 3

Showing 7 of 9 — disease pages carrying at least 10 antigens.

ReferencesPubMed · IEDB

Publications for NOVA1 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.18

gnomAD pLI

1

gnomAD missense Z

2.32

DepMap mean gene effect

0.04

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• mRNA splicing, via spliceosome
• negative regulation of cold-induced thermogenesis
• nervous system development
• regulation of alternative mRNA splicing, via spliceosome
• RNA processing
• RNA splicing

Molecular functions

• mRNA 3'-UTR binding
• mRNA binding
• RNA binding
• sequence-specific mRNA binding

Cellular components

• cytoplasm
• nucleolus
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• K Homology domain
• K Homology domain, type 1
• K Homology domain, type 1 superfamily
• RNA-binding protein Nova-1/2, type I K Homology domain 3
• RNA-binding protein Nova-1/2, type I K Homology domain 1
• RNA-binding protein Nova-1/2, type I K Homology domain 2
• KH domain

KeywordsUniProt

• mRNA processing
• mRNA splicing
• Neurogenesis
• Nucleus
• Phosphoprotein
• Repeat
• RNA-binding

InteractionsUniProt · HPA

Protein binding partners of NOVA1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• PTBP2
• HTR6