NME4

Protein identityUniProt · HPA

UniProt accession

O00746

Gene

NME4

Ensembl

ENSG00000103202

Chromosome

16

Canonical length

187 aa

Protein class

Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Mitochondria

Quaternary structure

Homohexamer

OverviewNCBI Gene

The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4 (Milon et al., 1997 [PubMed 9099850]).[supplied by OMIM, May 2008]

Canonical amino-acid sequenceUniProt

187 residues, UniProt reviewed canonical sequence.

>O00746|NME4
     1  MGGLFWRSAL RGLRCGPRAP GPSLLVRHGS GGPSWTRERT LVAVKPDGVQ RRLVGDVIQR
    61  FERRGFTLVG MKMLQAPESV LAEHYQDLRR KPFYPALIRY MSSGPVVAMV WEGYNVVRAS
   121  RAMIGHTDSA EAAPGTIRGD FSVHISRNVI HASDSVEGAQ REIQLWFQSS ELVSWADGGQ
   181  HSSIHPA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NME4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.39

Highest tissue expression

187 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.57

gnomAD pLI

0

gnomAD missense Z

-0.74

DepMap mean gene effect

0.05

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• CTP biosynthetic process
• GTP biosynthetic process
• intermembrane phospholipid transfer
• lipid transport
• nucleoside metabolic process
• protein hexamerization
• UTP biosynthetic process

Molecular functions

• ATP binding
• cardiolipin binding
• GTPase binding
• metal ion binding
• mitochondrion-mitochondrion outer membrane tether activity
• nucleoside diphosphate kinase activity
• phosphatidylglycerol transfer activity
• phospholipid binding
• protein-containing complex binding

Cellular components

• mitochondrial inner membrane
• mitochondrial intermembrane space
• mitochondrial matrix
• mitochondrial outer membrane
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• Nucleoside diphosphate kinase
• Nucleoside diphosphate kinase, active site
• Nucleoside diphosphate kinase-like domain
• Nucleoside diphosphate kinase-like domain superfamily
• Nucleoside diphosphate kinase

KeywordsUniProt

• ATP-binding
• Kinase
• Lipid-binding
• Magnesium
• Membrane
• Metal-binding
• Mitochondrion
• Mitochondrion outer membrane
• Nucleotide metabolism
• Nucleotide-binding
• Transferase
• Transit peptide

InteractionsUniProt · HPA

Protein binding partners of NME4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CAPN8
• OPA1
• NME1
• NME2
• NME3
• NXF1