Seroatlas · Human Serome Atlas

NEK8

Serine/threonine-protein kinase Nek8

Also known as: NEK8_HUMAN, NPHP9

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q86SG6
Gene
NEK8
Ensembl
ENSG00000160602
Chromosome
17
Canonical length
692 aa
Protein class
Disease related genes, Enzymes, Human disease related genes, Potential drug targets, Predicted intracellular proteins
Subcellular location
Nuclear speckles,Microtubules,Mitotic spindle,Primary cilium

OverviewNCBI Gene

This gene encodes a member of the serine/threionine protein kinase family related to NIMA (never in mitosis, gene A) of Aspergillus nidulans. The encoded protein may play a role in cell cycle progression from G2 to M phase. Mutations in the related mouse gene are associated with a disease phenotype that closely parallels the juvenile autosomal recessive form of polycystic kidney disease in humans. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

692 residues, UniProt reviewed canonical sequence.

>Q86SG6|NEK8
     1  MEKYERIRVV GRGAFGIVHL CLRKADQKLV IIKQIPVEQM TKEERQAAQN ECQVLKLLNH
    61  PNVIEYYENF LEDKALMIAM EYAPGGTLAE FIQKRCNSLL EEETILHFFV QILLALHHVH
   121  THLILHRDLK TQNILLDKHR MVVKIGDFGI SKILSSKSKA YTVVGTPCYI SPELCEGKPY
   181  NQKSDIWALG CVLYELASLK RAFEAANLPA LVLKIMSGTF APISDRYSPE LRQLVLSLLS
   241  LEPAQRPPLS HIMAQPLCIR ALLNLHTDVG SVRMRRAEKS VAPSNTGSRT TSVRCRGIPR
   301  GPVRPAIPPP LSSVYAWGGG LGTPLRLPML NTEVVQVAAG RTQKAGVTRS GRLILWEAPP
   361  LGAGGGSLLP GAVEQPQPQF ISRFLEGQSG VTIKHVACGD FFTACLTDRG IIMTFGSGSN
   421  GCLGHGSLTD ISQPTIVEAL LGYEMVQVAC GASHVLALST ERELFAWGRG DSGRLGLGTR
   481  ESHSCPQQVP MPPGQEAQRV VCGIDSSMIL TVPGQALACG SNRFNKLGLD HLSLGEEPVP
   541  HQQVEEALSF TLLGSAPLDQ EPLLSIDLGT AHSAAVTASG DCYTFGSNQH GQLGTNTRRG
   601  SRAPCKVQGL EGIKMAMVAC GDAFTVAIGA ESEVYSWGKG ARGRLGRRDE DAGLPRPVQL
   661  DETHPYTVTS VSCCHGNTLL AVRSVTDEPV PP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NEK8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.27
Highest tissue expression
8.2 nTPM

Expression across tissuesHPA

Tissue

  • kidney: 8.2 nTPM
  • thyroid gland: 6.9 nTPM
  • spleen: 5.6 nTPM
  • choroid plexus: 5.4 nTPM
  • lymph node: 5.4 nTPM
  • liver: 5.1 nTPM

Single-cell type

  • pdcs: 121 nCPM
  • epididymal basal cells: 50 nCPM
  • colonocytes: 35 nCPM
  • endometrial glandular cells: 32 nCPM
  • urothelial cells: 30 nCPM
  • enterocytes: 29 nCPM

Immune cell

  • plasmacytoid DC: 15 nTPM
  • naive B-cell: 4.2 nTPM
  • basophil: 3.3 nTPM
  • memory B-cell: 2.8 nTPM
  • NK-cell: 1.3 nTPM
  • eosinophil: 1.2 nTPM

Brain region

  • choroid plexus: 8.2 nTPM
  • white matter: 4.8 nTPM
  • medulla oblongata: 4.4 nTPM
  • cerebral cortex: 4.3 nTPM
  • thalamus: 4.2 nTPM
  • basal ganglia: 4.1 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about NEK8.

Disease | AllUniProt

Conditions NEK8 is implicated in, by any mechanism.

Disease | GeneticClinVar

40 pathogenic / likely-pathogenic of 500 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.8
gnomAD pLI
0
gnomAD missense Z
1.15
DepMap mean gene effect
-0.13
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NEK8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NEK8 as an antibody target. Whether an autoantibody or antibody against NEK8 could matter depends on whether native NEK8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NEK8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NEK8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NEK8. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...