Seroatlas · Human Serome Atlas

NDUFB5

NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial

Also known as: CI-SGDH, MGC12314, NDUB5_HUMAN, SGDH

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O43674
Gene: NDUFB5
Ensembl: ENSG00000136521
Chromosome: 3
Canonical length: 189 aa
Protein class: Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location: Nucleoplasm,Mitochondria

OverviewNCBI Gene

The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Canonical amino-acid sequenceUniProt

189 residues, UniProt reviewed canonical sequence.

>O43674|NDUFB5
     1  MAAMSLLRRV SVTAVAALSG RPLGTRLGFG GFLTRGFPKA AAPVRHSGDH GKRLFVIRPS
    61  RFYDRRFLKL LRFYIALTGI PVAIFITLVN VFIGQAELAE IPEGYVPEHW EYYKHPISRW
   121  IARNFYDSPE KIYERTMAVL QIEAEKAELR VKELEVRKLM HVRGDGPWYY YETIDKELID
   181  HSPKATPDN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NDUFB5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.56
Highest tissue expression: 226 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 226 nTPM
tongue: 156 nTPM
heart muscle: 123 nTPM
choroid plexus: 114 nTPM
duodenum: 71 nTPM
kidney: 63 nTPM

Single-cell type

late primary spermatocytes: 189 nCPM
early spermatids: 80 nCPM
parietal cells: 62 nCPM
choroid plexus epithelial cells: 60 nCPM
other brain neurons: 41 nCPM
brain inhibitory neurons: 37 nCPM

Immune cell

non-classical monocyte: 156 nTPM
myeloid DC: 146 nTPM
intermediate monocyte: 128 nTPM
total PBMC: 106 nTPM
classical monocyte: 105 nTPM
basophil: 95 nTPM

Brain region

choroid plexus: 63 nTPM
cerebral cortex: 47 nTPM
hypothalamus: 35 nTPM
thalamus: 34 nTPM
cerebellum: 33 nTPM
white matter: 31 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.26
gnomAD pLI: 0
gnomAD missense Z: -0.01
DepMap mean gene effect: -0.33
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 11% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

NADH dehydrogenase (ubiquinone) activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit
NADH:ubiquinone oxidoreductase, NDUFB5/SGDH subunit

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NDUFB5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NDUFB5 as an antibody target. Whether an autoantibody or antibody against NDUFB5 could matter depends on whether native NDUFB5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NDUFB5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NDUFB5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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