NDUFB6

Protein identityUniProt · HPA

UniProt accession

O95139

Gene

NDUFB6

Ensembl

ENSG00000165264

Chromosome

9

Canonical length

128 aa

Protein class

Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins

Subcellular location

Nucleoplasm,Mitochondria

OverviewNCBI Gene

The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jan 2011]

Canonical amino-acid sequenceUniProt

128 residues, UniProt reviewed canonical sequence.

>O95139|NDUFB6
     1  MTGYTPDEKL RLQQLRELRR RWLKDQELSP REPVLPPQKM GPMEKFWNKF LENKSPWRKM
    61  VHGVYKKSIF VFTHVLVPVW IIHYYMKYHV SEKPYGIVEK KSRIFPGDTI LETGEVIPPM
   121  KEFPDQHH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NDUFB6 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.52

Highest tissue expression

158 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.67

gnomAD pLI

0

gnomAD missense Z

-0.29

DepMap mean gene effect

-0.32

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• aerobic respiration
• mitochondrial ATP synthesis coupled electron transport
• mitochondrial electron transport, NADH to ubiquinone
• proton motive force-driven mitochondrial ATP synthesis

Molecular functions

• NADH dehydrogenase (ubiquinone) activity

Cellular components

• mitochondrial inner membrane
• mitochondrial membrane
• mitochondrion
• nucleoplasm
• respiratory chain complex I

Protein domainsUniProt · Pfam · InterPro

• NADH dehydrogenase 1, beta subcomplex, subunit 6
• NADH:ubiquinone oxidoreductase, NDUFB6/B17 subunit

KeywordsUniProt

• Acetylation
• Electron transport
• Membrane
• Mitochondrion
• Mitochondrion inner membrane
• Respiratory chain
• Transmembrane
• Transmembrane helix
• Transport

InteractionsUniProt · HPA

Protein binding partners of NDUFB6 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• NDUFA9
• NDUFB3
• NDUFB5