Seroatlas · Human Serome Atlas

MPHOSPH8

M-phase phosphoprotein 8

Also known as: HSMPP8, mpp8, MPP8_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q99549
Gene
MPHOSPH8
Ensembl
ENSG00000196199
Chromosome
13
Canonical length
860 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Plasma membrane,Cytosol
Quaternary structure
Homodimer

OverviewNCBI Gene

Enables chromatin binding activity; histone reader activity; and methylated histone binding activity. Involved in negative regulation of gene expression via chromosomal CpG island methylation and transposable element silencing by heterochromatin formation. Located in several cellular components, including cytosol; heterochromatin; and nuclear lumen. Part of nucleosome. [provided by Alliance of Genome Resources, Apr 2025]

Canonical amino-acid sequenceUniProt

860 residues, UniProt reviewed canonical sequence.

>Q99549|MPHOSPH8
     1  MEQVAEGARV TAVPVSAADS TEELAEVEEG VGVVGEDNDA AARGAEAFGD SEEDGEDVFE
    61  VEKILDMKTE GGKVLYKVRW KGYTSDDDTW EPEIHLEDCK EVLLEFRKKI AENKAKAVRK
   121  DIQRLSLNND IFEANSDSDQ QSETKEDTSP KKKKKKLRQR EEKSPDDLKK KKAKAGKLKD
   181  KSKPDLESSL ESLVFDLRTK KRISEAKEEL KESKKPKKDE VKETKELKKV KKGEIRDLKT
   241  KTREDPKENR KTKKEKFVES QVESESSVLN DSPFPEDDSE GLHSDSREEK QNTKSARERA
   301  GQDMGLEHGF EKPLDSAMSA EEDTDVRGRR KKKTPRKAED TRENRKLENK NAFLEKKTVP
   361  KKQRNQDRSK SAAELEKLMP VSAQTPKGRR LSGEERGLWS TDSAEEDKET KRNESKEKYQ
   421  KRHDSDKEEK GRKEPKGLKT LKEIRNAFDL FKLTPEEKND VSENNRKREE IPLDFKTIDD
   481  HKTKENKQSL KERRNTRDET DTWAYIAAEG DQEVLDSVCQ ADENSDGRQQ ILSLGMDLQL
   541  EWMKLEDFQK HLDGKDENFA ATDAIPSNVL RDAVKNGDYI TVKVALNSNE EYNLDQEDSS
   601  GMTLVMLAAA GGQDDLLRLL ITKGAKVNGR QKNGTTALIH AAEKNFLTTV AILLEAGAFV
   661  NVQQSNGETA LMKACKRGNS DIVRLVIECG ADCNILSKHQ NSALHFAKQS NNVLVYDLLK
   721  NHLETLSRVA EETIKDYFEA RLALLEPVFP IACHRLCEGP DFSTDFNYKP PQNIPEGSGI
   781  LLFIFHANFL GKEVIARLCG PCSVQAVVLN DKFQLPVFLD SHFVYSFSPV AGPNKLFIRL
   841  TEAPSAKVKL LIGAYRVQLQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MPHOSPH8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.54
Highest tissue expression
45 nTPM

Expression across tissuesHPA

Tissue

  • ovary: 45 nTPM
  • blood vessel: 42 nTPM
  • spleen: 38 nTPM
  • colon: 38 nTPM
  • lymph node: 37 nTPM
  • cerebellum: 36 nTPM

Single-cell type

  • ovarian stromal cells: 269 nCPM
  • leydig cells: 256 nCPM
  • differentiating spermatogonia: 250 nCPM
  • oocytes: 235 nCPM
  • epididymal principal cells: 233 nCPM
  • epididymal efferent duct absorptive cells: 215 nCPM

Immune cell

  • basophil: 134 nTPM
  • neutrophil: 69 nTPM
  • memory B-cell: 65 nTPM
  • naive B-cell: 54 nTPM
  • naive CD4 T-cell: 43 nTPM
  • eosinophil: 43 nTPM

Brain region

  • cerebral cortex: 73 nTPM
  • hypothalamus: 71 nTPM
  • midbrain: 63 nTPM
  • cerebellum: 63 nTPM
  • white matter: 62 nTPM
  • thalamus: 56 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.53
gnomAD pLI
0
gnomAD missense Z
1.08
DepMap mean gene effect
-0.29
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MPHOSPH8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MPHOSPH8 as an antibody target. Whether an autoantibody or antibody against MPHOSPH8 could matter depends on whether native MPHOSPH8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MPHOSPH8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MPHOSPH8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MPHOSPH8. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...