Seroatlas · Human Serome Atlas

MKRN2

E3 ubiquitin-protein ligase makorin-2

Also known as: HSPC070, MKRN2_HUMAN, RNF62

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9H000
Gene
MKRN2
Ensembl
ENSG00000075975
Chromosome
3
Canonical length
416 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Cytosol

OverviewNCBI Gene

This gene encodes a probable E3 ubiquitin ligase containing several zinc finger domains, that is a member of the makorin RING zinc-finger protein family. This gene overlaps the v-raf-1 murine leukemia viral oncogene homolog 1 (RAF1) gene in an antisense orientation and may have a co-regulatory function with RAF1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]

Canonical amino-acid sequenceUniProt

416 residues, UniProt reviewed canonical sequence.

>Q9H000|MKRN2
     1  MSTKQITCRY FMHGVCREGS QCLFSHDLAN SKPSTICKYY QKGYCAYGTR CRYDHTRPSA
    61  AAGGAVGTMA HSVPSPAFHS PHPPSEVTAS IVKTNSHEPG KREKRTLVLR DRNLSGMAER
   121  KTQPSMVSNP GSCSDPQPSP EMKPHSYLDA IRSGLDDVEA SSSYSNEQQL CPYAAAGECR
   181  FGDACVYLHG EVCEICRLQV LHPFDPEQRK AHEKICMLTF EHEMEKAFAF QASQDKVCSI
   241  CMEVILEKAS ASERRFGILS NCNHTYCLSC IRQWRCAKQF ENPIIKSCPE CRVISEFVIP
   301  SVYWVEDQNK KNELIEAFKQ GMGKKACKYF EQGKGTCPFG SKCLYRHAYP DGRLAEPEKP
   361  RKQLSSQGTV RFFNSVRLWD FIENRESRHV PNNEDVDMTE LGDLFMHLSG VESSEP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MKRN2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.48
Highest tissue expression
47 nTPM

Expression across tissuesHPA

Tissue

  • testis: 47 nTPM
  • heart muscle: 31 nTPM
  • pancreas: 25 nTPM
  • skeletal muscle: 25 nTPM
  • tongue: 23 nTPM
  • parathyroid gland: 23 nTPM

Single-cell type

  • late spermatids: 1,169 nCPM
  • early spermatids: 722 nCPM
  • cardiomyocytes: 204 nCPM
  • late primary spermatocytes: 131 nCPM
  • epicardial cells: 86 nCPM
  • myonuclei: 83 nCPM

Immune cell

  • eosinophil: 33 nTPM
  • NK-cell: 28 nTPM
  • MAIT T-cell: 26 nTPM
  • T-reg: 25 nTPM
  • non-classical monocyte: 23 nTPM
  • naive CD4 T-cell: 23 nTPM

Brain region

  • white matter: 19 nTPM
  • cerebellum: 18 nTPM
  • thalamus: 18 nTPM
  • hypothalamus: 17 nTPM
  • spinal cord: 17 nTPM
  • cerebral cortex: 16 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.66
gnomAD pLI
0.01
gnomAD missense Z
0.52
DepMap mean gene effect
-0.02
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MKRN2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MKRN2 as an antibody target. Whether an autoantibody or antibody against MKRN2 could matter depends on whether native MKRN2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MKRN2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MKRN2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MKRN2. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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